The aim of this brief report is to offer a solution for a problem that compromises the quality of in vitro-produced mammalian embryos. The harmful effects of evaporation-induced osmotic changes in mammalian embryo cultures have been recognized only recently. In this technical report, we describe a modified embryo culture dish (Humdish) that provides consistent >97% humidity and fully eliminates osmotic changes in the commonly used drop-under-oil culture systems from day 0 to 6. As an additional benefit, the Humdish also increases the temperature stability of cultures. If subsequent laboratory and clinical experiments prove its value, our suggested approach may help to improve the in vitro environment and quality of all preimplantation stage mammalian embryos, including the most sensitive ones produced from artificial gametes or by somatic cell nuclear transfer.

中文翻译：

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消除哺乳动物胚胎培养中蒸发的简单有效的解决方案

这份简短报告的目的是为影响体外生产的哺乳动物胚胎质量的问题提供解决方案。直到最近才认识到哺乳动物胚胎培养物中蒸发引起的渗透变化的有害影响。在本技术报告中，我们描述了一种改良的胚胎培养皿 (Humdish)，它提供一致的 >97% 湿度并完全消除常用的油底培养系统从第 0 天到第 6 天的渗透变化。作为一个额外的好处， Humdish 还增加了培养物的温度稳定性。如果随后的实验室和临床实验证明其价值，我们建议的方法可能有助于改善体外所有植入前哺乳动物胚胎的环境和质量，包括由人工配子或体细胞核移植产生的最敏感的胚胎。