ABSTRACT

Chronic ultraviolet (UV) exposure causes skin injury thorough the production of reactive oxygen species (ROS). Skin dryness, a characteristic of UV-induced injury, is closely related to skin barrier dysfunction. We investigated the effect of fermented black rice and blueberry with Lactobacillus plantarum (FBBBR) on UVB-induced skin injury. In vitro, FBBBR suppressed UVB-induced ROS generation, apoptosis, and metalloproteinase-9 expression, while increasing the expression of collagen type 1 alpha 1 (COL1A1) gene and natural moisturising factor-related genes such as filaggrin (FLG) and transglutaminase-1 (TGM-1). Oral administration of FBBBR increased serum catalase activity and decreased serum IgE levels, improved stratum corneum hydration and epidermal thickness and enhanced FLG, TGM-1, involucrin, and COL1A1 mRNA expression in dorsal skin in UVB-irradiated hairless mice. Therefore, we suggest that FBBBR can be used as a functional food with beneficial effects on skin hydration and skin barrier function.

摘要

慢性紫外线 (UV) 暴露会通过产生活性氧 (ROS) 导致皮肤损伤。皮肤干燥是紫外线损伤的一个特征，与皮肤屏障功能障碍密切相关。我们研究了发酵黑米和蓝莓与植物乳杆菌(FBBBR) 对 UVB 诱导的皮肤损伤的影响。在体外，FBBBR 抑制 UVB 诱导的 ROS 生成、细胞凋亡和金属蛋白酶-9 表达，同时增加胶原 1 型 α1 ( COL1A1 ) 基因和天然保湿因子相关基因如丝聚蛋白 ( FLG ) 和转谷氨酰胺酶-1 的表达。 ( TGM-1）。口服 FBBBR 可增加血清过氧化氢酶活性并降低血清 IgE 水平，改善角质层水合作用和表皮厚度，并增强UVB 照射的无毛小鼠背部皮肤中的FLG、TGM-1、外皮蛋白和COL1A1 mRNA 表达。因此，我们建议 FBBBR 可用作功能性食品，对皮肤水合作用和皮肤屏障功能具有有益作用。