Chitosan (85% deacetylated, viscosity > 400 MPa, and molecular weight of 570.3 kDa)/squid gelatin hydrolysates (SGH) were prepared by incorporating SGHs (10%, 20%, and 40%) into chitosan films. SGH were obtained from squid skin gelatin by hydrolysis with Alcalase. The effects of adding SGH on the physical, chemical structure, mechanical, degradability, antioxidant, and antifungal properties of the chitosan films were evaluated. Films containing SGH were opaquer and more colored than the reference. Scanning electron microscope imaging showed that the surface sections of the CH/SGH films were smooth and homogeneous, though a small amount of insoluble microparticles was observed. Atomic force microscopy indicated that the surface roughness of the chitosan films increased with the addition of SGH. Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy suggested an excellent compatibility of the components due to hydrogen bonding. The flexibility and in vitro degradability of the films increased as the SGH content increased. The 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate acid and 1,1-diphenyl-2-picrylhydrazyl scavenging rate of films increased with the addition of SGH. Moreover, films containing 20% SGH improved the fungistatic activity against Aspergillus parasiticus of chitosan films. The chitosan/SGH composite films have the potential for use in food packaging.

中文翻译：

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壳聚糖/鱿鱼皮明胶水解物薄膜的开发：结构、物理、抗氧化和抗真菌特性

壳聚糖（85% 脱乙酰，粘度 > 400 MPa，分子量为 570.3 kDa）/鱿鱼明胶水解物 (SGH) 通过将 SGH（10%、20% 和 40%）掺入壳聚糖薄膜中制备。SGH 是从鱿鱼皮明胶通过 Alcalase 水解获得的。评估了添加 SGH 对壳聚糖薄膜的物理、化学结构、力学、降解性、抗氧化和抗真菌性能的影响。含有 SGH 的薄膜比参照物更不透明且颜色更深。扫描电子显微镜成像显示 CH/SGH 薄膜的表面截面光滑均匀，但观察到少量不溶性微粒。原子力显微镜表明壳聚糖薄膜的表面粗糙度随着 SGH 的加入而增加。傅里叶变换红外光谱和核磁共振光谱表明，由于氢键，这些组分具有极好的相容性。随着 SGH 含量的增加，薄膜的柔韧性和体外降解性也随之增加。2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate acid)和1,1-diphenyl-2-picrylhydrazyl对薄膜的清除率随着SGH的加入而增加。此外，含有20% SGH的薄膜提高了抑菌活性反对壳聚糖薄膜的寄生曲霉。壳聚糖/SGH 复合薄膜具有用于食品包装的潜力。