Plant-specific BURP domain-containing proteins have an essential role in the plant's development and stress responses. Although BURP domain-containing proteins have been identified in several plant species, genome-wide analysis of the BURP gene family has not been investigated in the common bean. In the present study, we identified 11 BURP family members in the common bean (Phaseolus vulgaris) genome with a comprehensive in silico analysis. Pairwise alignment and phylogenetic analyses grouped PvBURP members into four subfamilies [RD-22 like (3), PG1β-like (4), BNM2-like (3), and USP-like (1)] according to their amino acid motifs, protein domains and intron–exon structure. The physical and biochemical characteristics of amino acids, motif and intron–exon structure, and cis-regulatory elements of BURPs members were determined. Promoter regions of BURP members included stress, light, and hormone response-related cis-elements. Therefore, expression profiles of PvBURP genes were identified with in silico tools and qRT-PCR analyses under stress (salt and drought) and hormone treatment (ABA, IAA) in the current study. While significant activity changes were not observed in BURP genes in RNA-seq data sets related to salt stress, it was determined that some BURP genes were expressed differently in those with drought stress. We identified 12 different miRNA, including miRNA395, miRNA156, miRNA169, miRNA171, miRNA319, and miRNA390, targeting the nine PvBURP genes using two different in silico tools based on perfect or near‐perfect complementarity to their targets. Here we present the first study to identify and characterize the BURP genes in common bean using whole-genome analysis, and the findings may serve as a reference for future functional research in common bean.

植物特有的含有 BURP 结构域的蛋白质在植物的发育和胁迫反应中具有重要作用。尽管已在多种植物物种中鉴定出含有 BURP 结构域的蛋白质，但尚未在普通豆中研究 BURP 基因家族的全基因组分析。在本研究中，我们通过全面的计算机分析，在普通菜豆 ( Phaseolus vulgaris ) 基因组中鉴定了 11 个 BURP 家族成员。配对比对和系统发育分析根据其氨基酸基序、蛋白质将PvBURP成员分为四个亚家族 [RD-22 样 (3)、PG1β 样 (4)、BNM2 样 (3) 和 USP 样 (1)]结构域和内含子-外显子结构。确定了 BURP 成员的氨基酸、基序和内含子-外显子结构以及顺式调控元件的物理和生化特征。BURP 成员的启动子区域包括与压力、光和激素反应相关的顺式元件。因此，在本研究中，通过计算机工具和 qRT-PCR 分析在胁迫（盐和干旱）和激素处理（ABA、IAA）下鉴定了PvBURP基因的表达谱。虽然在与盐胁迫相关的 RNA-seq 数据集中没有观察到 BURP 基因的显着活性变化，但确定一些 BURP 基因在干旱胁迫下的表达不同。我们鉴定了 12 种不同的 miRNA，包括 miRNA395、miRNA156、miRNA169、miRNA171、miRNA319 和 miRNA390，基于与靶标的完美或近乎完美的互补性，使用两种不同的计算机工具靶向 9 个PvBURP基因。在此，我们提出了第一个利用全基因组分析来鉴定和表征蚕豆中BURP基因的研究，该研究结果可以为未来蚕豆功能研究提供参考。