Bacterial pathogens are a major risk to human, animal, and plant health. To counteract the spread of antibiotic resistance, alternative antibacterial strategies are urgently needed. Here, we construct a proof-of-concept customizable, modular, and inducible antibacterial toxin delivery platform. By engineering a type VI secretion system (T6SS) that is controlled by an externally induced on/off switch, we transform the safe bacterium, Vibrio natriegens, into an effective antibacterial weapon. Furthermore, we demonstrate that the delivered effector repertoire, and thus the toxicity range of this platform, can be easily manipulated and tested. We believe that this platform can serve as a foundation for novel antibacterial bio-treatments, as well as a unique tool to study antibacterial toxins.

中文翻译：

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在 natriegens 中设计一个可定制的基于 T6SS 的抗菌平台

细菌病原体是人类、动物和植物健康的主要风险。为了对抗抗生素耐药性的传播，迫切需要替代的抗菌策略。在这里，我们构建了一个概念验证的可定制、模块化和可诱导的抗菌毒素递送平台。通过设计由外部感应开/关开关控制的 VI 型分泌系统 (T6SS)，我们将安全细菌Vibrio natriegens转化为有效的抗菌武器。此外，我们证明了交付的效应器曲目，以及该平台的毒性范围，可以很容易地操作和测试。我们相信该平台可以作为新型抗菌生物治疗的基础，以及研究抗菌毒素的独特工具。