Tilia miqueliana is in the genus Tilia (Tiliaceae [part of the Malvaceae per APGIII]) of the Malvales family. The mechanisms responsible for dormancy in T. miqueliana were investigated by exploring changes in seed coat permeability, seed water and endosperm content, and endogenous hormones during dormancy release and germination. The structure and permeability of the seed coat and endosperm were assessed using magnetic resonance imaging, scanning electron microscopy, and paraffin section detection. The biological activities of key enzymes in the oxidation pathway were evaluated. Soluble sugar and starch content was determined using the anthrone colorimetric method. Protein contents were determined using Coomassie Brilliant Blue G-250 staining, and the Soxhlet extraction method was used to determine fat content. The endogenous hormones abscisic acid (ABA), gibberellic acid (GA), and zeatin riboside (ZR) contents were determined using enzyme-linked immunosorbent assays. The results indicate that poor permeability and mechanical constraints in the seed coat and endosperm are the key mechanisms regulating dormancy in T. miqueliana seeds. The dormancy types are physical and physiological dormancy. Concentrated sulfuric acid (H₂SO₄) + gibberellic acid 3 (GA₃) + cold stratification treatments rapidly induced dormancy and accelerated germination. Activation of the tricarboxylic acid cycle and pentose phosphate pathway were critical for respiration. The metabolism of starch, protein, and fat in the endosperm was accelerated. The expression of endogenous GA₃, which promotes germination, increased, whereas that of ABA, which inhibits germination, decreased. The ratios of GA₃/ABA and ZR/ABA increased. Our study provides a theoretical foundation for the development of breeding methods.

南京椴是属中的椴树的锦葵目家族（椴树科[每APGIII锦葵科的一部分]）。T. miqueliana休眠的机制通过探索休眠解除和萌发过程中种皮渗透性、种子水分和胚乳含量以及内源激素的变化进行了研究。使用磁共振成像、扫描电子显微镜和石蜡切片检测评估种皮和胚乳的结构和渗透性。评价了氧化途径中关键酶的生物活性。可溶性糖和淀粉含量用蒽酮比色法测定。蛋白质含量采用考马斯亮蓝G-250染色法测定，脂肪含量采用索氏提取法测定。内源性激素脱落酸 (ABA)、赤霉酸 (GA) 和玉米素核苷 (ZR) 含量使用酶联免疫吸附测定法测定。T. miqueliana种子。休眠类型是物理休眠和生理休眠。浓硫酸 (H ₂ SO ₄ ) + 赤霉酸 3 (GA ₃ ) + 冷分层处理迅速诱导休眠并加速发芽。三羧酸循环和磷酸戊糖途径的激活对呼吸作用至关重要。加速胚乳中淀粉、蛋白质和脂肪的代谢。促进发芽的内源性GA ₃的表达增加，而抑制发芽的ABA的表达减少。GA ₃ /ABA 和ZR/ABA的比值增加。我们的研究为育种方法的发展提供了理论基础。