In this research, a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane environment. Excellent physicochemical properties of this novel monolith that were achieved included column efficiency, stability, and permeability. Moreover, the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small molecules. In particular, it exhibited good potential as an alternative to the commercial immobilized artificial membrane (IAM) column (IAM.PC.DD2) for studying drug-membrane interactions. This study not only enriched the types of IAM stationary phases, but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates.

本研究通过原位共聚 1-dodecanoyl-2-(11-methacrylamidoundecanoyl) -sn制备了一种新的基于磷脂的整体材料。-glycero-3-phosphoethanolamine 和二甲基丙烯酸乙酯模拟生物膜环境。这种新型整体材料具有出色的物理化学性质，包括柱效、稳定性和渗透性。此外，仿生整体材料对一系列完整蛋白质和小分子具有出色的分离能力。特别是，它表现出作为商业固定化人工膜 (IAM) 柱 (IAM.PC.DD2) 研究药物-膜相互作用的替代品的良好潜力。该研究不仅丰富了IAM固定相的种类，而且为预测候选药物的磷脂酰乙醇胺相关性质提供了一个简单的模型。