Self-healing hydrogel as a soft material with high durability and life-time has been successfully applied in various fields, including electronic skins, wearable electronic devices, and soft sensors. However, it is still a challenge to design a hydrogel with rapid self-healing, biodegradable and biosensing properties. Here, a self-healing carboxymethyl chitosan (CMCS)/oxidized carboxymethyl cellulose (OCMC) hydrogel with fluorescent bioprobes was developed for glucose detection. In this biosensing system, gold nanoclusters (AuNCs) and glucose oxidase (GOx) were encapsulated into the CMCS/OCMC hydrogel matrix as the fluorescent bioprobes. The CMCS/OCMC hydrogel with fluorescent bioprobes exhibited high sensitivity for glucose sensing with a linearly detection range of 100 μM to 5 mM and a detection limit of 0.029 mM, which covered the level of glucose in clinical detection. Furthermore, this hydrogel exhibited good biocompatibility. Finally, In vitro blood fluorescence tests and in vivo fluorescence investigation of the AuNCs-CMCS/OCMC hydrogel in diabetic mice indicated that this biocompatible and self-healing hydrogel based on fluorescent sensing system had potential application in implantable biosensing area for glucose monitoring.

自修复水凝胶作为一种具有高耐久性和使用寿命的软材料，已成功应用于电子皮肤、可穿戴电子设备和软传感器等各个领域。然而，设计一种具有快速自愈、可生物降解和生物传感特性的水凝胶仍然是一个挑战。在这里，开发了一种具有荧光生物探针的自修复羧甲基壳聚糖 (CMCS)/氧化羧甲基纤维素 (OCMC) 水凝胶，用于葡萄糖检测。在这个生物传感系统中，金纳米簇 (AuNCs) 和葡萄糖氧化酶 (GOx) 被封装到 CMCS/OCMC 水凝胶基质中作为荧光生物探针。具有荧光生物探针的 CMCS/OCMC 水凝胶对葡萄糖传感表现出高灵敏度，线性检测范围为 100 μM 至 5 mM，检测限为 0.029 mM，其中涵盖了临床检测中的葡萄糖水平。此外，这种水凝胶表现出良好的生物相容性。最后，AuNCs-CMCS/OCMC水凝胶在糖尿病小鼠体内的体外血液荧光试验和体内荧光研究表明，这种基于荧光传感系统的生物相容性和自愈性水凝胶在用于葡萄糖监测的植入式生物传感领域具有潜在应用。