A phytochemical investigation on the alkaloids from water-soluble part of Sophora alopecuroides led to obtain forty matrine-type alkaloids (1–40) including eighteen new ones (1–18), which covers almost all positions of the oxygen substitution in matrine-type structure. Notably, eight compounds (1–8) belong to rare bis-amide matrine-type alkaloid. The new structures were determined based on extensive spectroscopic data, electronic circular dichroism (ECD) calculations, and six instances, verified by X-ray crystallography. Most of isolates showed anti-neuroinflammatory activities based on the expression of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in BV2 microglia cells. Especially, compound 39 can suppress those two mediator secretions in a dose-dependent manner with IC₅₀ values of 21.6 ± 0.5 and 16.7 ± 0.8 μM, respectively. Further mechanistic study revealed that 39 suppressed the phosphorylation of IκBα and p65 subunit to regulate the NF-κB signaling pathway.

苦参水溶性部分生物碱的植物化学研究，获得苦参型生物碱40种（1 – 40），其中新的18种（1 – 18），几乎涵盖了苦参型氧取代的所有位置。结构体。值得注意的是，8种化合物（1 - 8）属于稀有双酰胺苦参碱型生物碱。新结构是基于广泛的光谱数据、电子圆二色性 (ECD) 计算和 X 射线晶体学验证的六个实例确定的。大多数分离株显示出基于肿瘤坏死因子 (TNF) -α的表达的抗神经炎症活性BV2 小胶质细胞中的白细胞介素 (IL)-6。特别是，化合物39可以以剂量依赖性方式抑制这两种介质的分泌，IC ₅₀值分别为 21.6 ± 0.5 和 16.7 ± 0.8 μM。此外机理研究揭示，39抑制I的磷酸化κ乙α和p65亚单位，以调节NF- κ乙信号传导途径。