ABSTRACT

India is the world’s 3rd largest shrimp producer at the same stint the shrimp industry generates 40%–70% waste of the catch volume. In this research, chitosan from shrimp (Penaeus indicus) waste was extracted at 75% degree of deacetylation and was brought down to powder form. Parallelly, a wet chemical precipitation method was adopted to produce hydroxyapatite suspension. The Chitosan powders were blended with the HAp suspension and finally ball milled to achieve composite powders of particle size 0.020 mm having mesoporous nature (3.17 nm) when quantified with BJH model. The scaffold was designed considering four design models namely the hollow cube, dumbbell, dual cone and honeycomb models with 62–67% of porosity. Post FE simulation analysis and design optimization using Pugh’s matrix revealed that the honeycomb model was superior considering the mass (g), porosity (%), compressive stress (MPa) and the level of complexity in mesh array. The composite powders were preheated up to 200°C and then the laser with spot of 0.5 mm width sinters the composite powder forming a scaffold as per design using the Selective Laser Sintering (SLS) process. The final part was subjected to cell viability assay using MG63 osteoblast cell line resulted in an increased cell level on the sixth day.

摘要

印度是世界第三大虾生产国，同时虾业产生了 40%–70% 的捕捞量浪费。在这项研究中，从虾 (Penaeus indicus) 废物中提取的壳聚糖在 75% 的脱乙酰度下被提取并制成粉末形式。同时，采用湿法化学沉淀法制备羟基磷灰石悬浮液。将壳聚糖粉末与 HAp 悬浮液混合，最后球磨得到粒径为 0.020 mm 的复合粉末，用 BJH 模型量化时具有中孔性质（3.17 nm）。支架的设计考虑了四种设计模型，即空心立方体、哑铃、双锥和蜂窝模型，孔隙率为 62-67%。使用 Pugh 矩阵进行的后 FE 模拟分析和设计优化表明，考虑到质量 (g)、孔隙率 (%)、压缩应力 (MPa) 和网格阵列的复杂程度，蜂窝模型更胜一筹。将复合粉末预热至 200°C，然后使用选择性激光烧结 (SLS) 工艺按照设计将具有 0.5 毫米宽光斑的激光烧结复合粉末形成支架。最后一部分使用MG63成骨细胞系进行细胞活力测定，导致第六天细胞水平增加。使用选择性激光烧结 (SLS) 工艺按照设计将 5 毫米宽的复合粉末烧结形成支架。最后一部分使用MG63成骨细胞系进行细胞活力测定，导致第六天细胞水平增加。使用选择性激光烧结 (SLS) 工艺按照设计将 5 毫米宽的复合粉末烧结形成支架。最后一部分使用MG63成骨细胞系进行细胞活力测定，导致第六天细胞水平增加。