Abstract

The bilayer nanofibrous membrane fabricated via electrospinning technique can be considered as an ideal structure for the treatment of chronic skin diseases and exudative wound dressings. Wound exudate would affect healing and increases the likelihood of infection at the same time. Therefore, it is essential to produce a kind of wound dressing with relatively high hygroscopicity which could absorb wound exudate and provide a relatively dry healing environment. Bilayer nanofibrous membranes of poly(L-lactide-co-ε-caprolactone)/tetracycline hydrochloride- polyethylene oxide/sodium alginate-zinc oxide (PLCL/TCH-PEO/SA-ZnO) with drug delivery potential were prepared by electrospinning for wound healing. Then, a cross-linking which involved soaking the samples in an aqueous solution containing strontium ions for 4 h was conducted. SEM images showed that membranes still maintained the peculiar nanofibrous structure. The spinning aid (PEO) used was removed in the cross-linked alginate without affecting the PLCL/TCH outer layer gave the membrane good mechanical properties and manageability. The hydrophilicity of the mats was tested to evaluate the ability of the bilayer membrane to absorb exudate from the wound. In vitro drug release suggested that antibacterial agents TCH could release continuously more than 10 days. The cross-linked fibrous membrane has improved mechanical properties and fluid repellency, thus representing a barrier to the external environment and effective wound protection. Consequently, the bilayer fibrous scaffold with good hygroscopicity and drug release properties would have wide applications prospects for the treatment of chronic skin diseases and exudative wound dressings.

摘要

双层纳米纤维膜通过静电纺丝技术可以被认为是治疗慢性皮肤病和渗出性伤口敷料的理想结构。伤口渗出液会影响愈合，同时增加感染的可能性。因此，有必要生产一种吸湿性较高、能吸收伤口渗出液并提供相对干燥的愈合环境的伤口敷料。静电纺丝制备具有药物递送潜力的聚(L-丙交酯-共-ε-己内酯)/盐酸四环素-聚环氧乙烷/海藻酸钠-氧化锌(PLCL/TCH-PEO/SA-ZnO)双层纳米纤维膜用于伤口愈合. 然后，进行交联，包括将样品浸泡在含有锶离子的水溶液中 4 小时。SEM图像显示，膜仍然保持着独特的纳米纤维结构。在不影响 PLCL/TCH 外层的情况下，在交联海藻酸盐中去除了所使用的纺丝助剂 (PEO)，从而使膜具有良好的机械性能和可管理性。测试垫子的亲水性以评估双层膜吸收伤口渗出物的能力。体外药物释放表明，抗菌药物TCH可以连续释放10天以上。交联纤维膜具有改善的机械性能和流体排斥性，因此代表了对外部环境的屏障和有效的伤口保护。因此，具有良好吸湿性和药物释放特性的双层纤维支架在治疗慢性皮肤病和渗出性伤口敷料方面具有广阔的应用前景。