Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a devastating disease of wheat in Turkey and worldwide. This pathogen can overcome known resistance genes and negatively affect the wheat production. The objectives of this study were to determine the virulence patterns of wheat stripe rust isolates collected from the coastal areas (Aegean and Mediterranean regions) of Turkey, and to examine the genetic diversity of the pathogen populations by simple sequence repeat (SSR) marker analysis. The majority of the isolates collected were virulent at various frequencies on the resistance genes Yr1 (50%), Yr6 (100%), Yr7 (78%), Yr8 (50%), Yr9 (84%), Yr10 (25%), Yr17 (38%), Yr24 (22%), Yr27 (31%), Yr32 (22%), Yr43 (47%), Yr44 (6%), YrSp (41%), YrTr1 (6%), and avirulent on Yr5 and Yr15. Based on the analysis of virulence, all isolates were determined as 25 races and clustered into six virulence groups (VGs). In contrast, the isolates clustered into four molecular groups (MGs) based on genotypic data. All four MGs were found in Aegean region of Turkey, while only three MGs (MG1, MG2 and MG3) were found in the Mediterranean region of the country. Additionally, analysis of molecular variance indicated that 80.9% of genetic variation was found within regions and 19.1% was found between the regions. The results suggest that interregional migration of the pathogen was high. The data can be helpful for the management of stripe rust and understanding the population structure and migration of Pst in Turkey.

摘要

条锈病，由Puccinia striiformis f.引起。sp. 小麦小麦是土耳其和世界范围内的一种毁灭性小麦病害。这种病原体可以克服已知的抗性基因并对小麦生产产生负面影响。本研究的目的是确定从土耳其沿海地区（爱琴海和地中海地区）收集的小麦条锈病分离株的毒力模式，并通过简单序列重复 (SSR) 标记分析检查病原体种群的遗传多样性。所收集的大多数分离株在上抗性基因的各种频率毒性YR1（50％），Yr6（100％），Yr7（78％），Yr8（50％），Yr9（84％），Yr10 (25%), Yr17 (38%), Yr24 (22%), Yr27 (31%), Yr32 (22%), Yr43 (47%), Yr44 (6%), YrSp (41%), YrTr1 ( 6%)，对Yr5和Yr15无毒. 根据毒力分析，所有分离株被确定为 25 个种族，并分为六个毒力组 (VG)。相比之下，根据基因型数据，分离株聚集成四个分子组 (MG)。在土耳其的爱琴海地区发现了所有 4 架 MG，而在该国的地中海地区仅发现了 3 架 MG（MG1、MG2 和 MG3）。此外，分子变异分析表明，80.9%的遗传变异发生在区域内，19.1%发生在区域之间。结果表明病原体的区域间迁移率很高。该数据有助于条锈病的管理和了解土耳其Pst的种群结构和迁移。