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How to Kinetically Dissect an RNA Machine
Biochemistry ( IF 2.9 ) Pub Date : 2021-09-07 , DOI: 10.1021/acs.biochem.1c00392
Rhiju Das 1, 2 , Rick Russell 3
Affiliation  

RNA-based machines are ubiquitous in Nature and increasingly important for medicines. They fold into complex, dynamic structures that process information and catalyze reactions, including reactions that generate new RNAs and proteins across biology. What are the experimental strategies and steps that are necessary to understand how these complex machines work? Two 1990 papers from Herschlag and Cech on “Catalysis of RNA Cleavage by the Tetrahymena thermophila Ribozyme” provide a master class in dissecting an RNA machine through kinetics approaches. By showing how to propose a kinetic framework, fill in the numbers, do cross-checks, and make comparisons across mutants and different RNA systems, the papers illustrate how to take a mechanistic approach and distill the results into general insights that are difficult to attain through other means.

中文翻译:


如何动力学解剖 RNA 机器



基于 RNA 的机器在自然界中无处不在,并且对药物越来越重要。它们折叠成复杂的动态结构,处理信息并催化反应,包括在生物学中产生新 RNA 和蛋白质的反应。了解这些复杂机器如何工作所需的实验策略和步骤是什么? Herschlag 和 Cech 于 1990 年发表的两篇论文“嗜热四膜虫核酶对 RNA 裂解的催化”为通过动力学方法解剖 RNA 机器提供了大师课程。通过展示如何提出动力学框架、填写数字、进行交叉检查以及对突变体和不同 RNA 系统进行比较,这些论文说明了如何采用机械方法并将结果提炼为难以获得的一般见解通过其他方式。
更新日期:2021-09-07
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