Accurate Identification and Early Diagnosis of Osteosarcoma through CRISPR-Cas12a-Based Average Telomerase Activity Detection,ACS Synthetic Biology

当前位置： X-MOL 学术 › ACS Synth. Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Accurate Identification and Early Diagnosis of Osteosarcoma through CRISPR-Cas12a-Based Average Telomerase Activity Detection
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2021-09-08 , DOI: 10.1021/acssynbio.1c00389
Guojun Wei ₁ , Zhibing Peng ₂ , Jingsong Liu ₂ , Kun Yang ₂ , Chenglong Zhao ₂ , Wei Xie ₃ , Tianwen Huang ₄ , Jiafeng Liu ₄ , Jin Li ₅ , Gang An ₂

Affiliation

Sensitive and reliable analysis of telomerase activity is important for clinical diagnosis, therapy, and prognosis of osteosarcoma. Telomerase activity is a complicated concept including both the amount of active telomerases and the length of the telomerases extension product. Still, few of the strategies formerly proposed distinguish the two aspects of telomerase activity. Herein, we propose a novel CRISPR-Cas12a-based fluorescent sensing platform that can output signals of both the amounts of telomerase and length of telomerase extension products with the assistance of an elegantly designed stem-loop probe and CRISPR-Cas12a system. On this basis, we induced a novel index, average telomerase activity, for accurate cancer reporting. Through systematic laboratory and clinical experiments, we have demonstrated that average telomerase activity can accurately distinguish cancer cells and has the potential for osteosarcoma staging.

中文翻译：

基于CRISPR-Cas12a平均端粒酶活性检测骨肉瘤的准确识别和早期诊断

灵敏可靠的端粒酶活性分析对于骨肉瘤的临床诊断、治疗和预后具有重要意义。端粒酶活性是一个复杂的概念，包括活性端粒酶的数量和端粒酶延伸产物的长度。尽管如此，以前提出的策略很少能够区分端粒酶活性的两个方面。在此，我们提出了一种基于CRISPR-Cas12a的新型荧光传感平台，在设计精美的茎环探针和CRISPR-Cas12a系统的帮助下，可以输出端粒酶数量和端粒酶延伸产物长度的信号。在此基础上，我们引入了一个新的指标，即平均端粒酶活性，以准确报告癌症。通过系统的实验室和临床实验，我们证明平均端粒酶活性可以准确区分癌细胞，并具有用于骨肉瘤分期的潜力。

更新日期：2021-09-17

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11