Rationale and Objectives: the use of zebrafish (Danio rerio) as an animal model in scientific studies has increased due to its many advantages for research. The multimodal micro PET/SPECT/CT equipment was developed for preclinical studies that use hybrid images of small rodents. It is not clear in the literature whether this type of equipment can be used for studies of smaller animals. We evaluated the feasibility of using the CT modality of a multimodal PET/SPECT/CT equipment for preclinical studies with zebrafish. Materials and Methods: since the CT modality of the equipment is optimized for imaging of small rodents, structural changes were tested to adapt it to the use of zebrafish: alteration of the additional aluminum filter of the X-ray tube and use of an adapted stretcher of high density extended polystyrene (EPS). The appearance of artifacts, changes in spatial resolution, contrast and image uniformity were evaluated using phantoms. Results: the images were visually analyzed to verify which anatomical structures can be identified after the system adjustments. The best results were obtained with a 0.2 mm thick aluminum filter and the adapted EPS stretcher, for both the phantom and animal images. It was possible to identify diverse structures such as swimming bladders, digestive tract and bone structures. Conclusion: using the proposed adaptations, the CT modality of a multimodal PET/SPECT/CT equipment can be utilized to carry out preclinical studies with zebrafish as the animal model.

基本原理和目标：斑马鱼 ( Danio rerio ) 在科学研究中作为动物模型的使用有所增加，因为它具有许多研究优势。多模式微型 PET/SPECT/CT 设备专为使用小型啮齿动物混合图像的临床前研究而开发。文献中不清楚这种类型的设备是否可用于研究较小的动物。我们评估了使用多模式 PET/SPECT/CT 设备的 CT 模式对斑马鱼进行临床前研究的可行性。材料和方法：由于设备的 CT 模式针对小型啮齿动物的成像进行了优化，因此对结构变化进行了测试以使其适应斑马鱼的使用：更改 X 射线管的附加铝滤光片并使用经过调整的高密度扩展担架聚苯乙烯 (EPS)。伪影的出现、空间分辨率的变化、对比度和图像均匀性均使用体模进行评估。结果：对图像进行视觉分析，以验证在系统调整后可以识别哪些解剖结构。对于幻影和动物图像，使用 0.2 毫米厚的铝过滤器和改装的 EPS 担架获得了最佳结果。可以识别不同的结构，如膀胱、消化道和骨骼结构。结论： 使用建议的适应性，多模式 PET/SPECT/CT 设备的 CT 模式可用于以斑马鱼作为动物模型进行临床前研究。