Long-Wavelength AIE-Based Fluorescent Probes for Mitochondria-Targeted Imaging and Photodynamic Therapy of Hepatoma Cells,ACS Applied Bio Materials

当前位置： X-MOL 学术 › ACS Appl. Bio Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Long-Wavelength AIE-Based Fluorescent Probes for Mitochondria-Targeted Imaging and Photodynamic Therapy of Hepatoma Cells
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2021-09-07 , DOI: 10.1021/acsabm.1c00673
Dan Zhao ₁ , Hai-Hao Han ₁ , Ling Zhu ₁ , Fang-Zhou Xu ₁ , Xing-Yu Ma ₁ , Jia Li ₂ , Tony D James _{3,

4} , Yi Zang ₂ , Xiao-Peng He ₁ , Chengyun Wang ₁

Affiliation

With this research, we have developed two long-wavelength theranostic probes (DCMT and DCMC) with aggregation-induced emission (AIE)-based properties for image-guided photodynamic therapy (PDT) of hepatoma cells. Introduction of a triphenylamine or carbazole group to a dicyanomethylene-4H-pyran dye with long-wavelength fluorescence emission produces the AIE-based probes, which were subsequently modified with triphenyl-phosphonium cation for actively targeting the mitochondria of hepatoma cells. Solution-based experiments show that the probes exhibit a mixed photophysical mechanism of twisted-intramolecular charge transfer and AIE at different aggregation states. The molecular aggregation of the probes also leads to an enhanced ability for oxygen photosensitization, suggesting their potential for PDT of cancer cells. Our subsequent cell-based assays show that the probes localize in the mitochondria of hepatoma cells and the use of light leads to cell death through the intracellular production of reactive oxygen species.

中文翻译：

基于长波长 AIE 的荧光探针用于肝癌细胞的线粒体靶向成像和光动力治疗

通过这项研究，我们开发了两种具有基于聚集诱导发射 (AIE) 特性的长波长治疗诊断探针（ DCMT和DCMC ），用于肝癌细胞的图像引导光动力治疗 (PDT)。将三苯胺或咔唑基团引入二氰基亚甲基-4 H具有长波长荧光发射的-吡喃染料产生基于 AIE 的探针，随后用三苯基鏻阳离子对其进行修饰，以主动靶向肝癌细胞的线粒体。基于溶液的实验表明，探针在不同的聚集状态下表现出扭曲的分子内电荷转移和 AIE 的混合光物理机制。探针的分子聚集还导致氧光敏化能力增强，表明它们具有用于癌细胞PDT的潜力。我们随后的基于细胞的测定表明，探针定位于肝癌细胞的线粒体，光的使用通过细胞内产生活性氧导致细胞死亡。

更新日期：2021-09-20

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11