Organic amendment may be an effective management practice for alleviating soil degradation in continuous cropping systems. However, few studies focus on the effects of long-term organic amendments on soil microbial community and microbial nutrient status in continuous tobacco fields. Here, an 11-year field experiment was conducted to investigate the effects of organic amendments on plant and soil physiochemical properties, microbial communities, ecoenzymatic stoichiometry, and glomalin-related soil protein (GRSP) accumulation in a continuous tobacco field. Five treatments were applied: CK (no fertilizer); CF (chemical fertilizer only); CFS (chemical fertilizer plus corn straw); CFO (chemical fertilizer plus oilseed residues); CFM (chemical fertilizer plus composted pig manure). Organic amendments increased soil microbial abundances. Application of organic fertilizers significantly reduced gram-positive to gram-negative bacterial ratios, but significantly increased fungi/bacteria ratios, probably because gram-negative bacteria and fungi are the dominant decomposers of increased available nutrients and recalcitrant materials, respectively. Organic amendments significantly enhanced soil enzyme activities, except for the difference in β-1,4-N-acetylglucosaminidase, Leucine aminopeptidase, and polyphenol oxidase (PPO) activities between the CF and CFO. The lowest soil organic carbon (SOC) content in CFS among organic amendments treatments was probably because the positive priming effects of straw and the highest peroxidase and PPO activities increased stable SOC mineralization rates due to the high C:N:P ratio of nutrients input. Microbial activities were limited by carbon, nitrogen, and phosphorus in CFS, while carbon and phosphorus were the limiting factors in CK, CF, CFO, and CFM. Relative to CF, CFO significantly enhanced microbial carbon and phosphorus limitation, whereas CFM significantly decreased microbial carbon limitation. CFM had the highest GRSP content among treatments. Our results demonstrated that combined application of composted pig manure and chemical fertilizers is a consistently effective measure for alleviating soil degradation in continuous tobacco fields.

有机改良剂可能是缓解连作系统土壤退化的有效管理措施。然而，很少有研究关注长期有机改良剂对连续烟田土壤微生物群落和微生物养分状况的影响。在这里，进行了为期 11 年的田间试验，以研究有机改良剂对连续烟草田中植物和土壤理化特性、微生物群落、生态酶化学计量学和球藻素相关土壤蛋白质 (GRSP) 积累的影响。施用五个处理：CK（不施肥）；CF（仅限化肥）；CFS（化肥加玉米秸秆）；CFO（化肥加油籽残渣）；CFM（化肥加堆肥猪粪）。有机改良剂增加了土壤微生物丰度。施用有机肥料显着降低了革兰氏阳性菌与革兰氏阴性菌的比率，但显着增加了真菌/细菌的比率，这可能是因为革兰氏阴性菌和真菌分别是增加的可用养分和顽固物质的主要分解者。有机改良剂显着增强土壤酶活性，除了 β-1,4-N-乙酰氨基葡萄糖苷酶、亮氨酸氨基肽酶和 CF 和 CFO 之间的多酚氧化酶 (PPO) 活性。在有机改良剂处理中，CFS 中土壤有机碳 (SOC) 含量最低可能是因为秸秆的积极启动效应以及最高的过氧化物酶和 PPO 活性增加了稳定的 SOC 矿化率，这是由于养分输入的高 C:N:P 比。CFS中微生物活动受到碳、氮和磷的限制，而碳和磷是CK、CF、CFO和CFM的限制因素。相对于 CF，CFO 显着增强了微生物碳和磷的限制，而 CFM 显着降低了微生物碳限制。CFM 在处理中具有最高的 GRSP 含量。