Archives of Virology ( IF 2.5 ) Pub Date : 2021-08-06 , DOI: 10.1007/s00705-021-05171-2 Angelique K Asselin 1 , Kayvan Etebari 1 , Michael J Furlong 1 , Karyn N Johnson 1
Native Australian soldier flies, Inopus spp. (Diptera: Stratiomyidae), are agricultural pests of economic importance to the sugarcane industry. A screen of the salivary gland transcriptome of Inopus flavus (James) revealed the presence of viral RNA belonging to a potentially novel member of the family Dicistroviridae. The complete genome sequence consists of 9793 nucleotides with two open reading frames. The genome includes two potential internal ribosomal entry sites (IRESs): one within the 5’ UTR and the other in the intergenic region (IGR). Virus particles purified from infected larvae and visualised by electron microscopy were found to be icosahedral, non-enveloped, and 30 nm in diameter.
中文翻译:
来自水虻的一种新的类双顺反病毒,黄白蚁(双翅目:Stratiomyidae),一种甘蔗害虫
澳大利亚本土士兵苍蝇,Inopus spp 。(双翅目:Stratiomyidae)是对甘蔗工业具有重要经济意义的农业害虫。Inopus flavus (James)唾液腺转录组的筛选显示存在属于Dicistroviridae家族潜在新成员的病毒 RNA 。完整的基因组序列由 9793 个核苷酸组成,具有两个开放阅读框。基因组包括两个潜在的内部核糖体进入位点 (IRES):一个位于 5' UTR 内,另一个位于基因间区域 (IGR)。从受感染的幼虫中纯化并通过电子显微镜观察的病毒颗粒被发现是二十面体、无包膜且直径为 30 nm。