Tomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice were given tomatidine by intraperitoneal injection for 7 consecutive days, and then, lung injury was induced via intratracheal instillation of lipopolysaccharide (LPS). Tomatidine reduced inflammatory cytokine expressions in bronchoalveolar lavage fluid (BALF), attenuated neutrophil infiltration in the BALF and lung tissue, increased superoxide dismutase activity and glutathione levels, and alleviated myeloperoxidase expression in the lung tissue of mice with lung injury. Tomatidine also decreased inflammatory cytokine and chemokine gene expression in inflammatory lungs and attenuated the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B. Furthermore, tomatidine enhanced the production of heme oxygenase-1, decreased the secretion of inflammatory cytokines and chemokines in LPS-stimulated lung epithelial cells, and attenuated THP-1 monocyte adhesion. Our findings suggest that tomatidine attenuates oxidative stress and inflammation, improving acute lung injury in mice.

中文翻译：

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番茄碱改善急性肺损伤小鼠的肺部炎症

从绿色番茄中分离出的番茄碱可以改善细胞和动物实验中的炎症和氧化应激，并已被证明可以改善哮喘小鼠模型中的气道炎症。在这里，我们研究了番茄碱是否可以改善小鼠的急性肺损伤。小鼠连续7天腹腔注射番茄碱，然后通过气管内滴注脂多糖（LPS）诱导肺损伤。Tomatidine 可降低支气管肺泡灌洗液 (BALF) 中炎性细胞因子的表达，减弱 BALF 和肺组织中的中性粒细胞浸润，增加超氧化物歧化酶活性和谷胱甘肽水平，并减轻肺损伤小鼠肺组织中髓过氧化物酶的表达。番茄碱还降低了炎症性肺中炎性细胞因子和趋化因子基因的表达，减弱了丝裂原活化蛋白激酶和核因子 kappa B 的磷酸化。此外，番茄碱增强了血红素加氧酶 1 的产生，减少了 LPS 中炎性细胞因子和趋化因子的分泌-刺激肺上皮细胞，减弱 THP-1 单核细胞粘附。我们的研究结果表明，番茄碱可减轻氧化应激和炎症，改善小鼠的急性肺损伤。并减弱 THP-1 单核细胞粘附。我们的研究结果表明，番茄碱可减轻氧化应激和炎症，改善小鼠的急性肺损伤。并减弱 THP-1 单核细胞粘附。我们的研究结果表明，番茄碱可减轻氧化应激和炎症，改善小鼠的急性肺损伤。