Currently, wound treatment is an urgent task of medicine around the world. In the process of wound healing, various types of cells are involved under the control and regulation of cytokines and growth factors. Disruption of the synchronization process between the various types of cells and intercellular mediators involved in the restoration of tissue damage can lead to impaired healing and the development of chronic wounds. Photobiomodulation (PBM) therapy promotes platelet activation and aggregation, reduces inflammation and oxidative stress, and accelerates cell migration and proliferation. PBM also induces the production of the extracellular matrix and the release of key growth factors, thereby improving tissue regeneration and accelerating wound healing. The aim of our work was to study the effect of photobiomodulation therapy on the regulation of reparative processes in chronic wounds monitored by biomarkers and platelet aggregation activity. 54 Wistar rats were divided into three groups. Intact animals were not manipulated. In animals of the control and experimental groups, a chronic wound was simulated by reproducing the conditions of local hypoxia and microcirculation disorders. The wounds of the experimental group received PBM therapy. The device Lika-therapist M (Ukraine) was used in a continuous mode at a wavelength of 660 nm, an output power of 10 mW, and an energy density of 1 J/cm². The wounds of the animals in the control group were treated with sham. The animals were euthanized on days 3, 7, 14, and 28 after the surgery (6 animals, each from the control and experimental groups). Measurements of the levels of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-α), the basic fibroblast growth factor (bFGF), and reactive oxygen species (ROS) were carried out by ELISA. Results revealed the multidirectional effect of PBM therapy on the expression of the studied biomarkers. The results of the histological examination indicated a positive effect of PBM therapy with the applied parameters on the repair processes of chronic wounds. We concluded that the use of PBM therapy made it possible to regulate disturbances in reparative processes by modulating ROS, cytokines, and platelet aggregation activity.

中文翻译：

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光生物调节疗法对慢性伤口愈合过程调节作用的实验研究

目前，伤口治疗是全世界医学的一项紧迫任务。在伤口愈合过程中，各种类型的细胞都在细胞因子和生长因子的控制和调节下参与。参与组织损伤修复的各种类型细胞和细胞间介质之间的同步过程中断会导致愈合受损和慢性伤口的发展。光生物调节 (PBM) 疗法可促进血小板活化和聚集，减少炎症和氧化应激，并加速细胞迁移和增殖。PBM 还诱导细胞外基质的产生和关键生长因子的释放，从而促进组织再生和加速伤口愈合。我们工作的目的是研究光生物调节疗法对通过生物标志物和血小板聚集活性监测的慢性伤口修复过程的调节作用。54只Wistar大鼠分为三组。未操纵完整的动物。在对照组和实验组的动物中，通过再现局部缺氧和微循环障碍的条件来模拟慢性伤口。实验组创面接受PBM治疗。设备 Lika-therapist M（乌克兰）以连续模式使用，波长为 660 nm，输出功率为 10 mW，能量密度为 1 J/cm 在对照组和实验组的动物中，通过再现局部缺氧和微循环障碍的条件来模拟慢性伤口。实验组创面接受PBM治疗。设备 Lika-therapist M（乌克兰）以连续模式使用，波长为 660 nm，输出功率为 10 mW，能量密度为 1 J/cm 在对照组和实验组的动物中，通过再现局部缺氧和微循环障碍的条件来模拟慢性伤口。实验组创面接受PBM治疗。设备 Lika-therapist M（乌克兰）以连续模式使用，波长为 660 nm，输出功率为 10 mW，能量密度为 1 J/cm² . 对照组动物的伤口采用假手术处理。在手术后第 3、7、14 和 28 天对动物实施安乐死（6 只动物，每只来自对照组和实验组）。测量白细胞介素 6 (IL-6)、白介素 10 (IL-10)、肿瘤坏死因子 α (TNF- α) 的水平)、碱性成纤维细胞生长因子 (bFGF) 和活性氧 (ROS) 通过 ELISA 进行。结果揭示了 PBM 治疗对所研究生物标志物表达的多方面影响。组织学检查的结果表明 PBM 治疗对慢性伤口修复过程的应用参数具有积极作用。我们得出结论，使用 PBM 疗法可以通过调节 ROS、细胞因子和血小板聚集活性来调节修复过程中的干扰。