Influence of surface topography on PCL electrospun scaffolds for liver tissue engineering,Journal of Materials Chemistry B

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Influence of surface topography on PCL electrospun scaffolds for liver tissue engineering
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2021-08-26 , DOI: 10.1039/d1tb00789k
Yunxi Gao ₁ , Anthony Callanan ₁

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Severe liver disease is one of the most common causes of death globally. Currently, whole organ transplantation is the only therapeutic method for end-stage liver disease treatment, however, the need for donor organs far outweighs demand. Recently liver tissue engineering is starting to show promise for alleviating part of this problem. Electrospinning is a well-known method to fabricate a nanofibre scaffold which mimics the natural extracellular matrix that can support cell growth. This study aims to investigate liver cell responses to topographical features on electrospun fibres. Scaffolds with large surface depression (2 μm) (LSD), small surface depression (0.37 μm) (SSD), and no surface depression (NSD) were fabricated by using a solvent–nonsolvent system. A liver cell line (HepG2) was seeded onto the scaffolds for up to 14 days. The SSD group exhibited higher levels of cell viability and DNA content compared to the other groups. Additionally, the scaffolds promoted gene expression of albumin, with all cases having similar levels, while the cell growth rate was altered. Furthermore, the scaffold with depressions showed 0.8 MPa higher ultimate tensile strength compared to the other groups. These results suggest that small depressions might be preferred by HepG2 cells over smooth and large depression fibres and highlight the potential for tailoring liver cell responses.

中文翻译：

表面形貌对肝组织工程用 PCL 电纺支架的影响

严重肝病是全球最常见的死亡原因之一。目前，全器官移植是治疗终末期肝病的唯一治疗方法，但供体器官供不应求。最近，肝脏组织工程开始显示出缓解部分这一问题的希望。静电纺丝是一种众所周知的制造纳米纤维支架的方法，它模仿可以支持细胞生长的天然细胞外基质。本研究旨在研究肝细胞对电纺纤维地形特征的反应。使用溶剂-非溶剂系统制备了具有大表面凹陷（2μm）（LSD）、小表面凹陷（0.37μm）（SSD）和无表面凹陷（NSD）的支架。将肝细胞系 (HepG2) 接种到支架上长达 14 天。与其他组相比，SSD 组表现出更高水平的细胞活力和 DNA 含量。此外，支架促进了白蛋白的基因表达，所有病例都具有相似的水平，同时细胞生长速率发生了改变。此外，与其他组相比，带有凹陷的支架的极限拉伸强度高出 0.8 MPa。这些结果表明，与光滑和大的凹陷纤维相比，HepG2 细胞可能更喜欢小凹陷，并强调了调整肝细胞反应的潜力。

更新日期：2021-09-07

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