Since it was first proposed in 2007, inertial microfluidics has been extensively studied in terms of theory, design, fabrication, and application. In recent years, with the rapid development of microfabrication technologies, a variety of channel structures that can focus, concentrate, separate, and capture bioparticles or fluids have been designed and manufactured to extend the range of potential biomedical applications of inertial microfluidics. Due to the advantages of high throughput, simplicity, and low device cost, inertial microfluidics is a promising candidate for rapid sample processing, especially for large-volume samples with low-abundance targets. As an approach to cellular sample pretreatment, inertial microfluidics has been widely employed to ensure downstream cell analysis and detection. In this review, a comprehensive summary of the application of inertial microfluidics for high-throughput cell analysis and detection is presented. According to application areas, the recent advances can be sorted into label-free cell mechanical phenotyping, sheathless flow cytometric counting, electrical impedance cytometer, high-throughput cellular image analysis, and other methods. Finally, the challenges and prospects of inertial microfluidics for cell analysis and detection are summarized.

中文翻译：

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用于高通量细胞分析和检测的惯性微流体：综述

自 2007 年首次提出以来，惯性微流体在理论、设计、制造和应用方面得到了广泛的研究。近年来，随着微细加工技术的飞速发展，各种能够聚焦、浓缩、分离和捕获生物颗粒或流体的通道结构被设计和制造，以扩展惯性微流体的潜在生物医学应用范围。由于具有高通量、简单性和低设备成本的优点，惯性微流体是快速样品处理的有希望的候选者，特别是对于具有低丰度目标的大体积样品。作为细胞样品预处理的一种方法，惯性微流体已被广泛用于确保下游细胞分析和检测。在这次审查中，介绍了惯性微流体在高通量细胞分析和检测中的应用的综合总结。根据应用领域，最新进展可分为无标记细胞机械表型分析、无鞘流式细胞计数、电阻抗细胞仪、高通量细胞图像分析等方法。最后，总结了惯性微流体在细胞分析和检测方面的挑战和前景。