ABSTRACT

A modified cry9Aa2 gene under the transcriptional control of the CaMV 35S promoter was transferred to four potato cultivars (‘Iwa’, ‘Karaka’, ‘Pacific’ and ‘Red Rascal’) using Agrobacterium-mediated gene transfer. Field plots were established for 170 independently regenerated transgenic lines derived from 113 transformation events. Phenotypic changes such as stunted plants, reduced vigour and/or leaf puckering, were observed in 29% of these lines. Foliage from the remaining 117 lines inhibited larval growth of potato tuber moth (Phthorimaea operculella Zeller) compared with non-transgenic controls. A high correlation was observed between greenhouse- and field-grown foliage for insect resistance using a detached leaf bioassay. Independently regenerated lines recovered from the same transformation event exhibited similar insect resistance. However, these lines derived from the same transformed cell differed markedly in phenotypic appearance and tuber yield. The first shoot regenerated from each transformation event was equivalent to the non-transgenic control for yield traits in only eight transformation events. A further 19 transformation events exhibited agronomic performance equivalent to the non-transgenic control when the second or subsequent regenerated shoots were evaluated. In clonal crops, multiple shoots from each transformation event should be independently assessed to ensure recovery of high-performing transgenic lines.

摘要

使用农杆菌介导的基因转移，将在 CaMV 35S 启动子的转录控制下的修饰的cry 9Aa2基因转移到四个马铃薯品种（'Iwa'、'Karaka'、'Pacific' 和 'Red Rascal'） 。为来自 113 个转化事件的 170 个独立再生的转基因品系建立了田间地块。在 29% 的这些品系中观察到表型变化，例如植株发育不良、活力降低和/或叶片皱缩。其余 117 个品系的叶子抑制了马铃薯块茎蛾（Phthorimaea opculella）的幼虫生长Zeller) 与非转基因对照相比。使用离体叶生物测定法观察到温室和田间种植的叶子对昆虫抗性的高度相关性。从同一转化事件中恢复的独立再生系表现出相似的抗虫性。然而，这些源自同一转化细胞的品系在表型外观和块茎产量方面存在显着差异。从每个转化事件再生的第一个枝条相当于仅八个转化事件中产量性状的非转基因对照。当评估第二次或随后的再生芽时，另外 19 个转化事件表现出与非转基因对照相当的农艺性能。在克隆作物中，