当前位置: X-MOL 学术Anal. Bioanal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Magnetic beads–assisted fluorescence aptasensing approach based on dual DNA tweezers for detection of ochratoxin A and fumonisin B1 in wine and corn
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2021-09-06 , DOI: 10.1007/s00216-021-03635-7
Chenling Qu 1 , Luyang Zhao 1 , Xing He 1 , Songcheng Yu 2 , Min Wei 1
Affiliation  

A magnetic beads (MBs)–assisted fluorescence aptasensing approach based on dual DNA tweezers and magnetic separation was established for the detection of ochratoxin A (OTA) and fumonisin B1 (FB1). A dual DNA tweezers structure with four ends linked with fluorophores (FAM, ROX) and quenchers (BHQ1, BHQ2) was designed, and produced the high initial fluorescence signals because of the long spatial distance between FAM and BHQ1, ROX, and BHQ2. Bio-aptamer/anti-aptamer of OTA and bio-aptamer/anti-aptamer of FB1 were respectively annealed to form dsDNA, and immobilized to MBs coated with streptavidin (SA). With the existence of OTA and FB1, OTA and FB1 preferentially bound with their respective bio-aptamers, which made anti-aptamers dissociate from dsDNA coupled on MBs. After magnetic separation, the dissociated anti-aptamers reacted with dual DNA tweezers, respectively, which made DNA tweezers close and the fluorescence was quenched. The linear ranges of approach for OTA and FB1 detection were 0.05–20 ng/mL and 0.1–40 ng/mL, respectively. The limit of detection for OTA and FB1 was 0.029 ng/mL and 0.061 ng/mL. The prepared MBs-assisted fluorescence aptasensing approach was applied to detect OTA and FB1 in spiked red wine and corn samples, which showed good recoveries between 92 and 106%.



中文翻译:

基于双DNA镊子的磁珠辅助荧光适配传感方法检测葡萄酒和玉米中的赭曲霉毒素A和伏马菌素B1

建立了一种基于双 DNA 镊子和磁分离的磁珠 (MB) 辅助荧光适配传感方法,用于检测赭曲霉毒素 A (OTA) 和伏马菌素 B 1 (FB 1 )。设计了一种双 DNA 镊子结构,其四个末端与荧光团(FAM、ROX)和猝灭剂(BHQ 1、BHQ 2)相连,由于 FAM 和 BHQ 1、ROX 和 ROX之间的空间距离长,因此产生了高初始荧光信号总部2 . OTA 的生物适体/抗适体和 FB 1 的生物适体/抗适体分别退火形成 dsDNA,并固定到涂有链霉亲和素 (SA) 的 MBs 上。随着OTA和FB 1的存在, OTA 和 FB 1优先与它们各自的生物适配体结合,这使得抗适配体与偶联在 MB 上的 dsDNA 分离。磁分离后,解离的抗适配体分别与双DNA镊子反应,使DNA镊子闭合,荧光猝灭。OTA 和 FB 1检测方法的线性范围分别为 0.05-20 ng/mL 和 0.1-40 ng/mL。OTA 和 FB 1的检测限为 0.029 ng/mL 和 0.061 ng/mL。制备的 MBs 辅助荧光适配传感方法用于检测加标红酒和玉米样品中的OTA 和 FB 1,其回收率在 92% 到 106% 之间。

更新日期:2021-09-07
down
wechat
bug