The study aimed to isolate and characterize zinc ionophores from Terminalia bellirica fruit using a liposome assay and test its utility in H9c2 rat cardiomyoblasts cells subjected to hypoxia/reoxygenation. Ethyl acetate extract that exhibited zinc ionophore activity was resolved to yield three polyphenols that were characterized as epicatechin-3-gallate (ECG), epigallocatechin-3-gallate (EGCG) and epigallocatechin (EGC) by nuclear magnetic resonance and electrospray ionization-mass spectra. The polyphenols enhanced the uptake of zinc into the liposomes and increased FluoZin-3 fluorescence. These polyphenols in the presence of 10 μM ZnCl₂ enhanced the zinc import into H9c2 cells, whose intracellular zinc levels were otherwise lowered upon hypoxia/reoxygenation. EGCG proved to be more potent than ECG, which indeed was more effective than EGC in improving cellular zinc levels and in attenuating the apoptosis of H9c2 cells after hypoxia/reoxygenation injury. The polyphenols required zinc for anti-apoptotic effect. The cardioprotective effect is indeed due to enhanced zinc uptake mediated by these polyphenols.

该研究旨在使用脂质体测定法从榄仁树果实中分离和表征锌离子载体，并测试其在缺氧/再充氧的 H9c2 大鼠心肌细胞中的效用。表现出锌离子载体活性的乙酸乙酯提取物被解析为三种多酚，通过核磁共振和电喷雾电离质谱表征为表儿茶素 3-没食子酸酯 (ECG)、表没食子儿茶素-3-没食子酸酯 (EGCG) 和表没食子儿茶素 (EGC) . 多酚增强了脂质体对锌的吸收并增加了 FluoZin-3 荧光。这些多酚在 10 μM ZnCl ₂存在下增强了 H9c2 细胞的锌输入，否则其细胞内锌水平在缺氧/复氧时会降低。EGCG 被证明比 ECG 更有效，在提高细胞锌水平和减弱缺氧/复氧损伤后 H9c2 细胞的凋亡方面，ECG 确实比 EGC 更有效。多酚需要锌才能发挥抗细胞凋亡作用。心脏保护作用确实是由于这些多酚介导的锌吸收增强所致。