Objective

Long non-coding RNAs (lncRNAs) are implicated in cancer-related cellular behaviors. Our research aimed to explore the biological functions of lncRNA AL592284.1 (AL592284.1) in cervical cancer (CC).

Methods

qRT-PCR was performed to examine AL592284.1 expressions in cell lines and tumor specimens. To study the roles of AL592284.1 on malignant behaviors in both in vitro and in vivo, Loss-of-function assays were carried out. Besides, bioinformatics prediction and dual-luciferase reporter assays were performed to reveal the interaction among AL592284.1 and its target genes. The functions of the AL592284.1/miR-30a-5p/Vimentin axis in CC cells was clarified by rescue assays.

Results

We observed that the levels of AL592284.1 in CC were distinctly increased. Functional assays revealed that knockdown of AL592284.1 suppressed the proliferation, migration, invasion and EMT progress of CC cells. Luciferase reporter assay confirmed that miR-30a-5p/Vimentin regulatory axis is the direct downstream of AL592284.1. Rescue experiments indicated that AL592284.1 induced overexpression of Vimentin via sponging miR-30a-5p, resulting in the promotion of CC progression.

Conclusion

The present study proves that AL592284.1 plays an tumor-promotive role in CC via regulating the miR-30a-5p/Vimentin axis, and inhibition of AL592284.1 may pave the way for CC treatment.

中文翻译：

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LncRNA AL592284.1通过miR-30a-5p/Vimentin/EMT轴促进宫颈癌细胞增殖和转移

 客观的

长非编码 RNA (lncRNA) 与癌症相关的细胞行为有关。我们的研究旨在探索lncRNA AL592284.1 (AL592284.1)在宫颈癌(CC)中的生物学功能。

 方法

采用 qRT-PCR 检测细胞系和肿瘤标本中的 AL592284.1 表达。为了研究 AL592284.1 对体外和体内恶性行为的作用，进行了功能丧失测定。此外，还进行了生物信息学预测和双荧光素酶报告基因检测，以揭示AL592284.1与其靶基因之间的相互作用。通过救援测定阐明了 CC 细胞中 AL592284.1/miR-30a-5p/Vimentin 轴的功能。

 结果

我们观察到 CC 中 AL592284.1 的水平明显增加。功能分析显示，敲低 AL592284.1 可抑制 CC 细胞的增殖、迁移、侵袭和 EMT 进程。荧光素酶报告基因检测证实 miR-30a-5p/Vimentin 调节轴是 AL592284.1 的直接下游。拯救实验表明，AL592284.1 通过海绵 miR-30a-5p 诱导 Vimentin 过度表达，从而促进 CC 进展。

 结论

本研究证明AL592284.1通过调节miR-30a-5p/Vimentin轴在CC中发挥促肿瘤作用，抑制AL592284.1可能为CC治疗铺平道路。