Acid-sensing ion channels (ASICs) play significant roles in numerous neurological and pathological conditions, including pain. Although acid-induced nociception has been characterized previously in zebrafish, the contribution of ASICs in modulating pain-like behaviors is still unknown. Here, we investigated the role of amiloride, a nonselective ASICs blocker, in the negative modulation of specific behavioral responses in a zebrafish-based model of acute visceral pain. We verified that intraperitoneal injection (i.p.) of 0.25, 0.5, 1.0, and 2.0 mg/mL amiloride alone or vehicle did not change zebrafish behavior compared to saline-treated fish. Administration of 2.5% acetic acid (i.p.) elicited writhing-like response evidenced by the abnormal body curvature and impaired locomotion and motor activity. Attenuation of acetic acid-induced pain was verified at lower amiloride doses (0.25 and 0.5 mg/mL) whereas 1.0 and 2.0 mg/mL abolished pain-like responses. The protective effect of the highest amiloride dose tested was evident in preventing writhing-like responses and impaired locomotion and vertical activity. Collectively, amiloride antagonized abdominal writhing-like phenotype and aberrant behaviors, supporting the involvement of ASICs in a zebrafish-based model of acute visceral pain.

酸敏感离子通道 (ASIC) 在包括疼痛在内的许多神经和病理状况中发挥着重要作用。尽管以前在斑马鱼中已经描述了酸诱导的伤害感受，但 ASIC 在调节疼痛样行为中的作用仍然未知。在这里，我们研究了阿米洛利的作用，一种非选择性 ASICs 阻滞剂，在基于斑马鱼的急性内脏疼痛模型中对特定行为反应的负调节。我们证实，与盐水处理的鱼相比，单独的 0.25、0.5、1.0 和 2.0 mg/mL 阿米洛利或载体的腹膜内注射 (ip) 不会改变斑马鱼的行为。2.5% 乙酸 (ip) 的给药引起了类似扭动的反应，这由异常的身体弯曲和受损的运动和运动活动证明。在较低的阿米洛利剂量（0.25 和 0.5 mg/mL）下证实了乙酸引起的疼痛减轻，而 1.0 和 2.0 mg/mL 消除了疼痛样反应。测试的最高阿米洛利剂量的保护作用在防止扭动样反应和运动和垂直活动受损方面是明显的。集体，