With the ongoing depletion of fossil fuel resources and emerging environmental issues, increasing research effort is being dedicated to producing biofuels from renewable substrates. With its advantages over ethanol in terms of energy density, octane number, and hygroscopicity, isobutanol is considered a potential alternative to traditional gasoline. However, as wild-type microorganisms cannot achieve the production of isobutanol with high titers and yields, rational genetic engineering has been employed to enhance its production. Herein, we review the latest developments in the metabolic engineering of Escherichia coli for the production of isobutanol, including those related to the utilization of diverse carbon sources, balancing the redox state, improving isobutanol tolerance, and application of synthetic biology circuits and tools.

随着化石燃料资源的不断枯竭和新出现的环境问题，越来越多的研究工作致力于从可再生基质生产生物燃料。异丁醇在能量密度、辛烷值和吸湿性方面优于乙醇，被认为是传统汽油的潜在替代品。然而，由于野生型微生物无法实现高滴度和高产量的异丁醇生产，因此已采用合理的基因工程来提高其产量。在此，我们回顾了大肠杆菌代谢工程的最新进展 用于异丁醇的生产，包括与各种碳源的利用、平衡氧化还原状态、提高异丁醇耐受性以及合成生物学电路和工具的应用相关的那些。