Anthracnose, caused byColletotrichum lentis, is a devastating disease of lentil (Lens culinaris Medik.) in western Canada. Growing resistant lentil cultivars is the most cost-effective and environmentally friendly approach to prevent seed yield losses that can exceed 70%. To identify loci conferring resistance to anthracnose race 1 in lentil, biparental quantitative trait loci (QTL) mapping of two recombinant inbred line (RIL) populations was integrated with a genome-wide association study (GWAS) using 200 diverse lentil accessions from a lentil diversity panel. A major-effect QTL (qAnt1.Lc-3) conferring resistance to race 1 was mapped to lentil chromosome 3 and colocated on the lentil physical map for both RIL populations. Clusters of candidate nucleotide-binding leucine-rich repeat (NB-LRR) and other defense-related genes were uncovered within the QTL region. A GWAS detected 14 significant single nucleotide polymorphism (SNP) markers associated with race 1 resistance on chromosomes 3, 4, 5, and 6. The most significant GWAS SNPs on chromosome 3 supported qAnt1.Lc-3 and delineated a region of 1.6 Mb containing candidate resistance genes. The identified SNP markers can be directly applied in marker-assisted selection (MAS) to accelerate the introgression of race 1 resistance in lentil breeding.

中文翻译：

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通过整合连锁作图和全基因组关联研究鉴定小扁豆炭疽病1型抗性基因座

炭疽病是由扁豆炭疽菌引起的，是加拿大西部扁豆（ Lens culinaris Medik.）的一种毁灭性疾病。种植抗性扁豆品种是防止种子产量损失超过 70% 的最具成本效益和环境友好的方法。为了鉴定小扁豆中赋予炭疽病种族 1 抗性的基因座，将两个重组近交系 (RIL) 群体的双亲数量性状基因座 (QTL) 作图与使用来自小扁豆多样性的 200 个不同小扁豆种质的全基因组关联研究 (GWAS) 相结合控制板。主要效应 QTL ( qAnt 1 .Lc-3) 对种族 1 的抗性被映射到小扁豆染色体 3，并位于两个 RIL 种群的小扁豆物理图谱上。在 QTL 区域内发现了候选核苷酸结合富含亮氨酸重复序列 (NB-LRR) 和其他防御相关基因的簇。GWAS 在 3、4、5 和 6 号染色体上检测到 14 个与种族 1 抗性相关的显着单核苷酸多态性 (SNP) 标记。3 号染色体上最显着的 GWAS SNP 支持qAnt 1.Lc - 3 并描绘了一个 1.6 Mb 的区域含有候选抗性基因。鉴定出的 SNP 标记可直接应用于标记辅助选择 (MAS) 中，以加速小扁豆育种中 1 号抗性的基因渗入。