We describe a label-free proteomics protocol for the interrogation of the placental proteome. Step-by-step directions, including tissue cleanup and preparation, proteolytic digestion, nanoLC–MS/MS data collection and data analysis, are provided. The workflow has been applied toward exploring differential protein expression patterns in placentas from women who have been exposed to drugs during pregnancy relative to those who have not. We collected 20 tissue specimens, each representing a combination of spatially diverse sections across the placenta. These specimens were analyzed in the work described here, to survey information across the entire organ. This protocol can be scaled up or down as needed.

中文翻译：

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发现人类胎盘组织的蛋白质组学


我们描述了一种用于胎盘蛋白质组询问的无标记蛋白质组学方案。提供了分步指导，包括组织清理和制备、蛋白水解消化、nanoLC-MS/MS 数据收集和数据分析。该工作流程已应用于探索妊娠期间接触过药物的女性与未接触过药物的女性胎盘中的差异蛋白表达模式。我们收集了 20 个组织样本，每个样本代表胎盘空间不同切片的组合。在此描述的工作中对这些标本进行了分析，以调查整个器官的信息。该协议可以根据需要放大或缩小。