Protein allergens is a health risk for consumption of soybeans. To understand allerginicity mechanism, T cell epitopes of 7 soybean allergens were predicted and screened by abilities to induce cytokine interleukin (IL) 4. The relationships among amino acid composition, properties, allergenicity, and pepsin hydrolysis sites were analyzed. Among the 138 T cell epitopes identified, YIKDVFRVIPSEVLS, KDVFRVIPSEVLSNS, DVFRVIPSEVLSNSY of Gly m 6.0501 (P04347), and AKADALFKAIEAYLL, ADALFKAIEAYLLAH of Gly m 4.0101 (P26987) were the most possible epitope candidates. In T cell epitopes pattern, the frequencies of amino acids Q, D, E, P, and G decreased, while F, I, N, V, K, H, A, L, and S increased. Hydrophobic residues at positions p1 and p2 and positively charged residues in positions p13 might contribute to allergenicity. Most of epitopes could be hydrolyzed by pepsin into small polypeptides within 12 residues length, and the anti-digestive epitope regions contained I, V, S, N, and Q residues. T cell epitopes EEQRQQEGVIVELSK from Gly m 5.03 (P25974) showed resistance to pepsin hydrolysis and would cause a higher Th2 cell response. This research provides basis for the development of hypoallergenic soybean products in the soybean industry as well as for the immunotherapy design for protein allergy.

中文翻译：

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使用生物信息学方法预测和表征主要大豆蛋白过敏原的 T 细胞表位


蛋白质过敏原是食用大豆的健康风险。为了了解过敏机制，通过诱导细胞因子白细胞介素（IL）4的能力来预测和筛选7种大豆过敏原的T细胞表位。分析氨基酸组成、性质、过敏性和胃蛋白酶水解位点之间的关系。在鉴定的 138 个 T 细胞表位中，Gly m 6.0501 (P04347) 的 YIKDVFRVIPSEVLS、KDVFRVIPSEVLSNS、DVFRVIPSEVLSNSY 和 Gly m 4.0101 (P26987) 的 AKADALFKAIEAYLL、ADALFKAIEAYLLAH 是最可能的候选表位。在T细胞表位模式中，氨基酸Q、D、E、P和G的频率减少，而F、I、N、V、K、H、A、L和S的频率增加。 p1 和 p2 位点的疏水残基以及 p13 位点的带正电残基可能会导致过敏性。大多数表位可以被胃蛋白酶水解成12个残基长度内的小多肽，并且抗消化表位区域包含I、V、S、N和Q残基。来自 Gly m 5.03 (P25974) 的 T 细胞表位 EEQRQQEGVIVELSK 显示出对胃蛋白酶水解的抗性，并会引起更高的 Th2 细胞反应。该研究为大豆行业开发低过敏性大豆产品以及蛋白质过敏的免疫治疗设计提供依据。