Low-temperature plasma-jet-activated medium inhibited tumorigenesis of lung adenocarcinoma in a 3D in vitro culture model,Plasma Processes and Polymers

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Low-temperature plasma-jet-activated medium inhibited tumorigenesis of lung adenocarcinoma in a 3D in vitro culture model
Plasma Processes and Polymers ( IF 2.9 ) Pub Date : 2021-09-06 , DOI: 10.1002/ppap.202100049
Ying Li ₁ , Yang Lv ₂ , Mengxiong Tang ₃ , Eun H. Choi ₄ , Junjie Wang ₅ , Guoqing Lv ₆ , Yu Zhu ₃ , Shubin Wang ₃ , Yajie Liu ₁

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Many studies have shown, using two-dimensional culture, that low-temperature plasma-jet-activated medium (PAM) inhibits the growth of various tumor cells, whereas its effects and mechanisms in the three-dimensional (3D) culture system remain unclear. In this study, a 3D cell culture model was used to study the inhibition of tumorigenesis of PAM in vitro. Our results showed that PAM significantly suppressed tumor spheroid formation and downregulated stemness-related genes of A549 cells “before,” “during,” and “after” sphere formation. Additionally, we found that the specific signature of reactive oxygen species-induced autophagy was involved in the antiproliferation effect of PAM on A549 spheroids. Our study complemented and extended the application of PAM for its anticancer effects in lung adenocarcinoma cancer.

中文翻译：

低温等离子体喷射活化培养基在 3D 体外培养模型中抑制肺腺癌的肿瘤发生

许多研究表明，使用二维培养，低温等离子体喷射激活培养基（PAM）抑制各种肿瘤细胞的生长，而其在三维（3D）培养系统中的作用和机制尚不清楚。在这项研究中，使用 3D 细胞培养模型来研究体外 PAM 对肿瘤发生的抑制。我们的结果表明，PAM 在球体形成“之前”、“期间”和“之后”显着抑制肿瘤球体形成并下调 A549 细胞的干细胞相关基因。此外，我们发现活性氧诱导的自噬的特定特征与 PAM 对 A549 球体的抗增殖作用有关。我们的研究补充并扩展了 PAM 在肺腺癌中的抗癌作用的应用。

更新日期：2021-11-04

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