Lamb’s-ear (Stachys byzantina L.) serves as valuable medicinal plant mainly because of its pharmaceutical phyto-metabolites like linalool. Polyploidy induction has been known as an effective approach to enhance phyto-medicines content in various medicinal plants. To fulfill the production of S. byzantin with higher biomass and more secondary metabolites, different colchicine concentrations including 0.1, 0.2 and 0.4 % (w/v), were applied on the apical meristem for different periods including 12, 24, and 48 h, followed by chromosome counting and flow-cytometry to confirm the putative tetraploids, which had been remarked in preliminary morphological screening. The results exhibited that the chromosome number and DNA content successfully duplicated in tetraploids, 2n = 4x = 60 and 2 C DNA = 2.4 pg, when compared to diploids with 2n = 2x = 30 and 2 C DNA = 1.2 pg. A ploidy induction efficiency of ~ 18 % was achieved from the optimal treatment of 0.2 % colchicine for 12 h. The tetraploid plants had the larger and thicker leaves, as well as shorter stems and internodes. Stomata and trichomes of tetraploid plants were larger with lower density than those of diploid plants. The chlorophyll, linalool, α-cadinol, cubenol, α-terpineol, and menthone content significantly increased in tetraploids. Altogether, in vitro polyploidy can improve the important phyto-metabolites content in S. byzantin.

羊耳（Stachys byzantina L. ）作为有价值的药用植物主要是因为它的药用植物代谢物如芳樟醇。多倍体诱导被认为是提高各种药用植物中植物药含量的有效方法。完成S. byzantin的生产生物量更高，次生代谢物更多，不同浓度的秋水仙碱，包括 0.1、0.2 和 0.4 % (w/v)，在不同时期（包括 12、24 和 48 小时）应用于顶端分生组织，然后进行染色体计数和流式细胞术确认推定的四倍体，已在初步形态学筛选中注明。结果表明，与具有 2n = 2x = 30 和 2 C DNA = 1.2 pg 的二倍体相比，染色体数量和 DNA 含量在四倍体中成功复制，2n = 4x = 60 和 2 C DNA = 2.4 pg。0.2% 秋水仙碱最佳处理 12 小时的倍性诱导效率约为 18%。四倍体植物的叶子更大更厚，茎和节间更短。与二倍体植物相比，四倍体植物的气孔和毛状体较大，密度较低。四倍体中叶绿素、芳樟醇、α-卡地诺、苦参醇、α-萜品醇和薄荷酮的含量显着增加。共，体外多倍体可提高拜占庭沙门菌中重要植物代谢物的含量。