In this study, a bioactive composite material based on calcium sulfate hemihydrate (CSH) bone cement was studied, which use calcium sulfate dihydrate (CSD) as coagulant and silk fibroin nanofibers (SFF) solution as the curing liquid, further loaded vancomycin silk fibroin microspheres (SFM/VCM). The drug release effect of bone cements caused by tuning weight content of SFM/VCM (0.5, 1, 2%) and the concentration of silk fibroin solution (SFS) (20, 60, 100 mg/mL) used for preparation of SFM was studied in this article. Scanning electron microscope (SEM) demonstrated that the average diameter of microspheres gradually increased and the setting time was prolonged with the concentration of SFS increasing. X-ray diffraction (XRD) and Fourier transform infrared (FTIR) were used to analyze the composition of composite materials. The result of compressive strength revealed that the composites contained 0.5% SFM/VCM showed better mechanical performance independent on the concentration of microspheres and the cumulative drug release percentage of all composites were less than 55% after 4 weeks. The drug-loading bone cement possesses not only injectability but also sustained release capability which has a promising prospect in the field of bone substitute material.

中文翻译：

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硫酸钙骨水泥联合调谐丝素纳米纤维和万古霉素负载丝素微球的制备及性能研究

本研究研究了一种基于半水硫酸钙（CSH）骨水泥的生物活性复合材料，以二水硫酸钙（CSD）为凝固剂，丝素蛋白纳米纤维（SFF）溶液为固化液，进一步负载万古霉素丝素蛋白微球。 (SFM/VCM)。调整 SFM/VCM 的重量含量 (0.5, 1, 2%) 和用于制备 SFM 的丝素蛋白溶液 (SFS) 的浓度 (20, 60, 100 mg/mL) 对骨水泥的药物释放效果是本文研究。扫描电镜（SEM）表明，随着SFS浓度的增加，微球的平均直径逐渐增大，凝固时间延长。X射线衍射（XRD）和傅里叶变换红外（FTIR）用于分析复合材料的成分。抗压强度结果表明，含有 0.5% SFM/VCM 的复合材料表现出更好的力学性能，不受微球浓度的影响，4 周后所有复合材料的累积药物释放百分比均小于 55%。该载药骨水泥不仅具有可注射性，还具有缓释能力，在骨替代材料领域具有广阔的应用前景。