T cell homeostasis, T cell differentiation, and T cell effector function rely on the constant fine-tuning of gene expression. To alter the T cell state, substantial remodeling of the proteome is required. This remodeling depends on the intricate interplay of regulatory mechanisms, including post-transcriptional gene regulation. In this review, we discuss how the sequence of a transcript influences these post-transcriptional events. In particular, we review how sequence determinants such as sequence conservation, GC content, and chemical modifications define the levels of the mRNA and the protein in a T cell. We describe the effect of different forms of alternative splicing on mRNA expression and protein production, and their effect on subcellular localization. In addition, we discuss the role of sequences and structures as binding hubs for miRNAs and RNA-binding proteins in T cells. The review thus highlights how the intimate interplay of post-transcriptional mechanisms dictate cellular fate decisions in T cells.

中文翻译：

---

序列决定簇作为 T 细胞基因表达的关键调节因子

T 细胞稳态、T 细胞分化和 T 细胞效应功能依赖于基因表达的不断微调。为了改变 T 细胞状态，需要对蛋白质组进行大量重塑。这种重塑取决于调控机制复杂的相互作用，包括转录后基因调控。在这篇综述中，我们讨论了转录本的序列如何影响这些转录后事件。特别是，我们回顾了序列决定因素（例如序列保守性、GC 含量和化学修饰）如何定义 T 细胞中 mRNA 和蛋白质的水平。我们描述了不同形式的选择性剪接对 mRNA 表达和蛋白质产生的影响，以及它们对亚细胞定位的影响。此外，我们还讨论了序列和结构作为 T 细胞中 miRNA 和 RNA 结合蛋白结合中心的作用。因此，该综述强调了转录后机制的密切相互作用如何决定 T 细胞的细胞命运决定。