The fluorescence imaging and precise suppression of bacterial infections in chronic wounds by porphyrin-based metal–organic framework nanorods,Journal of Materials Chemistry B

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The fluorescence imaging and precise suppression of bacterial infections in chronic wounds by porphyrin-based metal–organic framework nanorods
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2021-08-17 , DOI: 10.1039/d1tb01649k
Lihua Zhang _{1,

2,

3} , Minzhi Ouyang ₄ , Yufei Zhang ₃ , Leiyi Zhang ₄ , Ziyun Huang ₃ , Libei He ₃ , Yanli Lei ₃ , Zhen Zou ₃ , Feng Feng _{1,

2} , Ronghua Yang _{3,

5}

Affiliation

Nano-antibacterial agents can play a critical role in chronic wound management. However, the design of an intelligent nanosystem that can provide both a visual warning of infection and precise sterilization remains a hurdle. Herein, a rod-like porphyrin-based metal–organic framework theranostic nanosystem (Zn-TCPP nanorods) is fabricated via coordination chelation between tetrakis(4-carboxylphenyl)porphyrin and zinc ions. This system can show significant fluorescence activation in response to the local elevated pH shown by chronic wounds, a main indicator of wound infection. Meanwhile, under the guidance of fluorescence imaging, the highly spatiotemporally precise photodynamic inactivation of microorganisms can be carried out without the destruction of surrounding normal cells and nascent cells. The results demonstrated that the Zn-TCPP nanorods were a highly sensitive and reversible probe for sensing alkaline pH levels. Alterations in the fluorescence of the Zn-TCPP nanorods can accurately indicate the infection status and heterogeneity of infection within the wound bed. Under specific light irradiation, the Zn-TCPP nanorods can exterminate 97% of Staphylococcus aureus via the generation of reactive oxygen species (ROS). Assays of extensive wounds demonstrate that the precise fluorescence-imaging-guided suppression of bacterial infection can significantly reduce the mouse mortality rate and accelerate wound healing. This system provides the opportunity for “precision medicine” relating to chronic wounds and some large-area wounds.

中文翻译：

卟啉基金属有机骨架纳米棒对慢性伤口细菌感染的荧光成像和精确抑制

纳米抗菌剂可以在慢性伤口管理中发挥关键作用。然而，设计既能提供感染视觉警告又能精确消毒的智能纳米系统仍然是一个障碍。在此，通过制备棒状卟啉基金属-有机框架治疗纳米系统（Zn-TCPP 纳米棒）四（4-羧基苯基）卟啉与锌离子之间的配位螯合。该系统可以响应慢性伤口显示的局部升高的 pH 值显示显着的荧光激活，这是伤口感染的主要指标。同时，在荧光成像的指导下，可以在不破坏周围正常细胞和新生细胞的情况下，对微生物进行高度时空精确的光动力灭活。结果表明，Zn-TCPP 纳米棒是一种用于检测碱性 pH 值的高灵敏度和可逆探针。Zn-TCPP 纳米棒荧光的变化可以准确地指示伤口床内的感染状态和感染的异质性。在特定光照射下，Zn-TCPP 纳米棒可以杀死 97%金黄色葡萄球菌通过产生活性氧 (ROS)。广泛伤口的测定表明，精确的荧光成像引导抑制细菌感染可以显着降低小鼠死亡率并加速伤口愈合。该系统为慢性伤口和一些大面积伤口的“精准医疗”提供了机会。

更新日期：2021-09-06

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