Highly sensitive and rapid detection of porcine circovirus 2 by avidin–biotin complex based lateral flow assay coupled to isothermal amplification,Analytical Methods

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Highly sensitive and rapid detection of porcine circovirus 2 by avidin–biotin complex based lateral flow assay coupled to isothermal amplification
Analytical Methods ( IF 2.7 ) Pub Date : 2021-08-31 , DOI: 10.1039/d1ay01189h
Minju Jang ₁ , SeJin Kim ₂ , Junkyu Song ₂ , Sanghyo Kim _{1,

2}

Affiliation

In this study, a new platform for the detection of porcine circovirus 2 was developed by avidin–biotin complex based lateral flow assay (LAMP-LFA). Improved detection sensitivity was attained by using loop mediated isothermal amplification (LAMP) with a low limit of detection (LOD), so the platform can be used to detect even early or asymptomatic stages of infection. LFA, which requires no specialized equipment, facilitates the use of point-of-care (POC) tests. Therefore, by applying LFA, the result can be confirmed accurately with the naked eye. Moreover, this platform has a unique structure using a single-tag detection system. The avidin–biotin interaction is the strongest interaction between proteins and has a higher K_d value than antigen–antibody interactions. Thus, the results are stable and can be clearly confirmed. The high sensitivity of LAMP-LFA enables all steps to be completed in 30 min. As a result, it could be applied to different targets, such as other pathogens. Future POC diagnostic studies are expected to be of great practical benefit.

中文翻译：

通过基于抗生物素蛋白-生物素复合物的侧向流动测定结合等温扩增来高灵敏度和快速检测猪圆环病毒 2

在这项研究中，通过基于抗生物素蛋白-生物素复合物的侧向流动测定法 (LAMP-LFA) 开发了检测猪圆环病毒 2 的新平台。通过使用低检测限 (LOD) 的环介导等温扩增 (LAMP) 提高了检测灵敏度，因此该平台可用于检测感染的早期或无症状阶段。LFA 不需要专门的设备，便于使用即时 (POC) 测试。因此，通过应用 LFA，可以用肉眼准确地确认结果。此外，该平台具有使用单标签检测系统的独特结构。亲和素-生物素相互作用是蛋白质之间最强的相互作用，具有更高的K _d比抗原-抗体相互作用更有价值。因此，结果是稳定的并且可以清楚地证实。LAMP-LFA 的高灵敏度使所有步骤能够在 30 分钟内完成。因此，它可以应用于不同的目标，例如其他病原体。预计未来的 POC 诊断研究将具有巨大的实际益处。

更新日期：2021-09-06

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