Ultrasensitive quantification of extracellular vesicles through dual signal amplification for the early diagnosis and prognosis of chronic obstructive pulmonary disease (COPD),Analytical Methods

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Ultrasensitive quantification of extracellular vesicles through dual signal amplification for the early diagnosis and prognosis of chronic obstructive pulmonary disease (COPD)
Analytical Methods ( IF 2.7 ) Pub Date : 2021-08-23 , DOI: 10.1039/d1ay01294k
Jingjun Xie ₁ , Can Wu _{2,

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Affiliation

Accurate quantification of low-abundant EVs plays an essential role in the diagnosis and treatment of chronic obstructive pulmonary disease (COPD). Aptamers, which can specifically recognize and bind with protein molecules through transformation, make it possible to integrate DNA polymerase-based amplification strategies for protein detection. Thus, we have designed an allosteric probe and demonstrated its feasibility to convert the detection signals of EVs (extracellular vesicles) to nucleic acids through the specific recognition of target EVs. In addition, we have integrated the Nt.BstNBI and DNA polymerase based ssDNA generation process with the Exo III recycle process and greatly improved the detection sensitivity. The presence of target EVs initiates the Nt.BstNBI triggered multiple cycle amplification, enabling the achievement of high sensitivity and excellent selectivity, holding great potential in disease diagnosis and biomedical research.

中文翻译：

通过双信号放大对细胞外囊泡进行超灵敏定量，用于慢性阻塞性肺疾病 (COPD) 的早期诊断和预后

低丰度 EV 的准确定量在慢性阻塞性肺疾病 (COPD) 的诊断和治疗中起着至关重要的作用。适配体可以通过转化特异性识别和结合蛋白质分子，使得整合基于 DNA 聚合酶的扩增策略用于蛋白质检测成为可能。因此，我们设计了一种变构探针，并证明了其通过对目标 EV 的特异性识别将 EV（细胞外囊泡）的检测信号转化为核酸的可行性。此外，我们将基于 Nt.BstNBI 和 DNA 聚合酶的 ssDNA 生成过程与 Exo III 循环过程相结合，大大提高了检测灵敏度。目标 EV 的存在启动 Nt.BstNBI 触发多循环放大，

更新日期：2021-09-06

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