BSC2 induces multidrug resistance via contributing to the formation of biofilm in Saccharomyces cerevisiae,Cellular Microbiology

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BSC2 induces multidrug resistance via contributing to the formation of biofilm in Saccharomyces cerevisiae
Cellular Microbiology ( IF 2.6 ) Pub Date : 2021-09-05 , DOI: 10.1111/cmi.13391
Zhiwei Huang ₁ , Hongsheng Dai ₁ , Xiaoyu Zhang ₁ , Qiao Wang ₁ , Jing Sun ₂ , Yunxia Deng ₁ , Ping Shi ₃

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Biofilm plays an important role in fungal multidrug resistance (MDR). Our previous studies showed that BSC2 is involved in resistance to amphotericin B (AMB) through antioxidation in Saccharomyces cerevisiae. In this study, the overexpression of BSC2 and IRC23 induced strong MDR in S. cerevisiae. BSC2-overexpression affected cellular flocculation, cell surface hydrophobicity, biofilm formation and invasive growth. However, it failed to induce caspofungin (CAS) resistance and affect the invasive growth in FLO mutant strains (FLO11Δ, FLO1Δ, FLO8Δ and TUP1Δ). Furthermore, the overexpression of BSC2 compensated for chitin synthesis defects to maintain the cell wall integrity and significantly reduced the cell morphology abnormality induced by CAS. However, it could not repair the cell wall damage caused by CAS in the FLO mutant strains. Although BSC2 overexpression increased the level of mannose in the cell wall, DPM1 overexpression in both BY4741 and bsc2∆ could confer resistance to CAS and AMB. In addition, BSC2 overexpression significantly increased the mRNA expression of FLO11, FLO1, FLO8 and TUP1. BSC2 may function as a regulator of FLO genes and be involved in cell wall integrity in yeast. Taken together, our data demonstrate that BSC2 induces MDR in a FLO pathway-dependent manner via contributing to the formation of biofilms in S. cerevisiae.

中文翻译：

BSC2通过促进酿酒酵母生物膜的形成诱导多药耐药

生物膜在真菌多药耐药（MDR）中起着重要作用。我们之前的研究表明，BSC2通过酿酒酵母的抗氧化作用参与了对两性霉素 B (AMB) 的抗性。在这项研究中，BSC2和IRC23的过表达在S. cerevisiae中诱导了强烈的 MDR 。BSC2-过表达影响细胞絮凝、细胞表面疏水性、生物膜形成和侵入性生长。然而，它未能诱导卡泊芬净 (CAS) 抗性并影响FLO突变菌株 ( FLO11 Δ、FLO1 Δ、FLO8 Δ 和TUP1Δ)。此外，BSC2的过表达弥补了几丁质合成缺陷，以维持细胞壁的完整性，并显着降低了CAS诱导的细胞形态异常。然而，它不能修复FLO突变株中 CAS 引起的细胞壁损伤。尽管BSC2过表达增加了细胞壁中甘露糖的水平，但 BY4741 和bsc2 Δ 中的DPM1过表达可赋予对 CAS 和 AMB 的抗性。此外，BSC2过表达显着增加了FLO11、FLO1、FLO8和TUP1的mRNA表达。BSC2可能作为FLO基因的调节剂并参与酵母细胞壁的完整性。总之，我们的数据表明BSC2通过促进酿酒酵母中生物膜的形成以FLO途径依赖性方式诱导 MDR 。

更新日期：2021-09-05

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