A 60-d feeding trial was conducted to evaluate the effects of Saccharomyces cerevisiae and β-glucan on the growth performance, feed utilization and immunity response of Macrobrachium rosenbergii. Prawns (0.19 ± 0.01 g) were fed with the basal diet (group C) or other three experimental diets supplemented with S. cerevisiae (group S), β-glucan (group G) or their combination (group GS), respectively. Results showed that the growth performance of prawns was significantly promoted in group S and GS (p < 0.05). Immunostimulants supplementation significantly increased the protease activity and the highest amylase activity was found in group G (p < 0.05). The highest phagocytic index of prawns was observed in group GS (p < 0.05), and the respiratory burst activity was significantly improved in group S and GS (p < 0.05). Furthermore, immunostimulant supplementation significantly increased the gene expression of the antioxidant and antimicrobial enzymes of prawns (p < 0.05). Immunostimulant supplementation also significantly increased the gene expression of prophenoloxidase, toll-like receptors, heat shock protein 70 and lectins(p < 0.05). Conclusively, S. cerevisiae and β-glucan both could be used as potential immunostimulants in prawns farming. Considering the comprehensive effects, their combination was more effective in promoting the growth and immunity of prawns.

中文翻译：

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日粮酿酒酵母和β-葡聚糖对罗氏沼虾生长性能、抗氧化能力和免疫反应的影响

进行了 60 天的饲养试验，以评估酿酒酵母和 β-葡聚糖对罗氏沼虾生长性能、饲料利用率和免疫反应的影响。对虾 (0.19 ± 0.01 g) 分别喂食基础饮食（C 组）或其他三种添加了酿酒酵母（S 组）、β-葡聚糖（G 组）或它们的组合（GS 组）的实验饮食。结果表明，S组和GS组对虾的生长性能显着提高（p  < 0.05）。免疫刺激剂的补充显着增加了蛋白酶活性，G 组的淀粉酶活性最高（p < 0.05)。GS组对虾的吞噬指数最高（p  < 0.05），S组和GS组对虾的呼吸爆发活动明显改善（p <  0.05）。此外，免疫刺激剂的补充显着增加了对虾抗氧化和抗微生物酶的基因表达（p <  0.05）。补充免疫刺激剂还显着增加了酚氧化酶原、toll 样受体、热休克蛋白 70 和凝集素的基因表达（p <  0.05）。最终，酿酒酵母和 β-葡聚糖都可以用作对虾养殖的潜在免疫刺激剂。从综合作用来看，它们的组合更能有效地促进对虾的生长和免疫。