ABSTRACT

Limonium genus is traditionally used in North Africa for disease treatment and in cosmetic. This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense. Analysis of phenolic compounds was carried out by using RP-HPLC. Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined. Inhibiting of NO release in LPS-stimulated macrophages was assessed. L. cercinense exhibited strong antioxidant and anti-inflammatory effects. In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense. High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species. The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.

摘要

柠檬属传统上在北非用于疾病治疗和化妆品。本研究首次调查了两种突尼斯特有植物L. boitardii和L. cercinense的抗氧化、抗炎和酶抑制活性。酚类化合物的分析通过使用 RP-HPLC 进行。测定总酚和类黄酮含量、抗氧化活性（抗自由基、还原和总抗氧化活性）、酪氨酸酶和α-葡萄糖苷酶、胶原酶抑制活性。评估了 LPS 刺激的巨噬细胞中 NO 释放的抑制作用。L. cercinense具有很强的抗氧化和抗炎作用。在酪氨酸酶和α-葡萄糖苷酶抑制剂活性测试中，这两个物种的活性很高，尤其是L. cercinense。L. cercinense和杨梅苷、杨梅素、杨梅素 3 - O -β-D-galatopyranoside、木犀草素 7- O-葡萄糖苷和芦丁的总酚和类黄酮含量较高，是这两个物种的主要酚类物质。获得的结果使L. boitardii和L. cercinense成为化妆品和药理学应用的有价值的新天然来源。