A 2,4,6-trinitrophenol (TNP) degrading bacterial strain isolated from a site polluted with explosives was identified as Proteus sp. strain OSES2 via 16S rRNA gene sequencing. Metabolic investigation showed that the organism grew exponentially on 100 mg l⁻¹ of TNP as a source of carbon, nitrogen, and energy. In addition, the growth of the organism was sustainable on 3-nitrotoluene, 2,4-dinitrotoluene, 2,4,6-trinitrotoluene, 4-nitrophenol, methyl-3-nitrobenzoate, 4-nitroaniline, aniline and nitrobenzene. Strain OSES2 was able to utilize TNP within a concentration range of 100 mg l⁻¹ to 500 mg l⁻¹. The specific growth rate and degradation rates on TNP were 0.01043 h⁻¹ and 0.01766 mg l⁻¹ h⁻¹ respectively. Effective degradation of TNP in a chemically defined medium was evident with a gradual reduction in the concentration of TNP concomitant with an increase in cell density as well as the substantial release of ammonium (NH₄⁺), nitrite (NO₂⁻), and nitrate (NO₃⁻) as metabolites in 96 h. Degradation competence of the organism was enhanced in the presence of starch and acetate. On starch-supplemented TNP, the highest specific growth rate and degradation rates were 0.02634 h⁻¹ and 0.04458 mg l⁻¹ h⁻¹, respectively, while the corresponding values on acetate were 0.02341 h⁻¹ and 0.02811 mg l⁻¹ h⁻¹. However, amendment with nitrogen sources yielded no substantial improvement in degradation. TNP was utilized optimally at pH 7 to 9 and within the temperature range of 30 °C to 37 °C. The enzyme hydride transferase II [HTII], encoded by the npdI gene which is the first step involved in the TNP degradation pathway, was readily expressed by the isolate thus suggesting that substrate was utilized through the classical metabolic pathway.

从被炸药污染的地点分离出的一种 2,4,6-三硝基苯酚 (TNP) 降解菌株被鉴定为变形杆菌。通过 16S rRNA 基因测序菌株 OSES2。代谢研究表明，有机体在 100 mg l ^-1作为碳、氮和能量来源的 TNP 上呈指数增长。此外，有机体在3-硝基甲苯、2,4-二硝基甲苯、2,4,6-三硝基甲苯、4-硝基苯酚、3-硝基苯甲酸甲酯、4-硝基苯胺、苯胺和硝基苯上的生长是可持续的。^{菌株 OSES2 能够在 100 mg l -1}至 500 mg l ^-1的浓度范围内利用 TNP 。TNP的比生长速率和降解速率分别为0.01043 h ^-1和0.01766 mg l ^-1 h ^-1分别。TNP 在化学成分确定的培养基中的有效降解很明显，随着细胞密度的增加，TNP 的浓度逐渐降低，并且大量释放出铵 (NH ₄ ⁺ )、亚硝酸盐 (NO ₂ ^- ) 和硝酸盐(NO ₃ ^- ) 在 96 小时内作为代谢物。在淀粉和乙酸盐的存在下，生物体的降解能力增强。在添加淀粉的 TNP 上，最高的比生长速率和降解速率分别为 0.02634 h ^-1和 0.04458 mg l ^-1  h ^-1，而乙酸盐的相应值为 0.02341 h ^-1和 0.02811 mg l ^-1  h ^-1。然而，用氮源进行修正并没有对降解产生实质性的改善。TNP 在 pH 7 至 9 和 30 °C 至 37 °C 的温度范围内得到最佳利用。由npdI基因编码的氢化物转移酶 II [HTII]是参与 TNP 降解途径的第一步，很容易被分离物表达，因此表明通过经典代谢途径利用了底物。