Citrus tristeza virus was detected for the first time in the Lao People’s Democratic Republic. Samples were collected from citrus trees across the southern provinces for testing in Australia. RNA was extracted and tested using conventional and real-time reverse transcription polymerase chain reactions with the virus detected in 12 of 59 samples tested. Viral identities were confirmed by sequencing. Additional confirmation was obtained by an enzyme-linked immunosorbent assay. The implications of the presence of this virus for citrus production in Lao are discussed briefly.

中文翻译：

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老挝人民民主共和国首次报告柑橘tristeza病毒

老挝人民民主共和国首次检测到柑橘类病毒。从南部各省的柑橘树中收集样品，以便在澳大利亚进行测试。使用常规和实时逆转录聚合酶链反应提取 RNA，并在 59 个测试样本中的 12 个样本中检测到病毒。通过测序确认病毒身份。通过酶联免疫吸附试验获得了额外的确认。简要讨论了这种病毒的存在对老挝柑橘生产的影响。