Abstract

Context

Adipose tissue and physical inactivity both influence metabolic health and systemic inflammation, but how adipose tissue responds to chronic physical inactivity is unknown.

Objective

This work aimed to characterize the impact of chronic physical inactivity on adipose tissue in healthy, young males.

Methods

We collected subcutaneous adipose tissue from 20 healthy, young men before and after 60 days of complete bed rest with energy intake reduced to maintain energy balance and fat mass. We used RNA sequencing, flow cytometry, ex vivo tissue culture, and targeted protein analyses to examine adipose tissue phenotype.

Results

Our results indicate that the adipose tissue transcriptome, stromal cellular compartment, and insulin signaling protein abundance are largely unaffected by bed rest when fat mass is kept stable. However, there was an increase in the circulating concentration of several adipokines, including plasma leptin, which was associated with inactivity-induced increases in plasma insulin and absent from adipose tissue cultured ex vivo under standardized culture conditions.

Conclusion

Physical inactivity–induced disturbances to adipokine concentrations such as leptin, without changes to fat mass, could have profound metabolic implications outside a clinical facility when energy intake is not tightly controlled.

中文翻译：

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长期不运动对脂肪组织免疫代谢的影响

摘要

语境

脂肪组织和缺乏运动都会影响代谢健康和全身炎症，但脂肪组织对慢性缺乏运动的反应尚不清楚。

客观的

这项工作旨在描述慢性身体不活动对健康年轻男性脂肪组织的影响。

方法

我们收集了 20 名健康年轻男性在完全卧床 60 天前后的皮下脂肪组织，减少能量摄入以维持能量平衡和脂肪量。我们使用 RNA 测序、流式细胞术、离体组织培养和靶向蛋白质分析来检查脂肪组织表型。

结果

我们的结果表明，当脂肪量保持稳定时，脂肪组织转录组、基质细胞区室和胰岛素信号蛋白丰度在很大程度上不受卧床休息的影响。然而，包括血浆瘦素在内的几种脂肪因子的循环浓度增加，这与不活动诱导的血浆胰岛素增加有关，并且在标准化培养条件下离体培养的脂肪组织中不存在。

结论

当能量摄入没有得到严格控制时，身体不活动引起的脂肪因子浓度（如瘦素）在不改变脂肪量的情况下可能会在临床设施之外产生深远的代谢影响。