Numerous studies have suggested that the abnormal expression of circular RNAs plays an essential role in the pathological progression of numerous tumors. Nonetheless, the functions and underlying mechanisms of the circular RNA circCRIM1 in osteosarcoma (OS) are still not fully understood. In this study, 47 classes of OS tissues and adjoining normal tissues were obtained from patients. Real-time PCR was employed to measure circCRIM1 expression levels in both OS tissues and cell lines. The proliferation, migration, and invasion ability in OS cell lines were measured by MTT assays, EDU assays, transwell migration experiments, and transwell invasion assays. The results demonstrated that the expression of circCRIM1 was notably decreased both in OS tissues and cell lines. Depressed circCRIM1 expression was correlated with lymph node metastasis, advanced FIGO stage, and low overall survival of OS patients. In addition, the results indicated that circCRIM1 could decrease the migration, invasion, and growth of OS cells. Further mechanistic studies indicated that circCRIM1 served as a competing endogenous RNA (ceRNA) of miR-513, leading to decreases in the proliferation, migration, and invasion of OS cells. Taken together, our data uncovered a significant role of the circCRIM1/miR-513 pathway in the proliferation, migration, and invasion of OS cell lines and suggested that circCRIM1 may serve as a possible therapeutic target for OS treatment.

大量研究表明，环状RNA的异常表达在众多肿瘤的病理进展中起着至关重要的作用。尽管如此，circRNA circCRIM1 在骨肉瘤（OS）中的功能和潜在机制仍未完全了解。在这项研究中，从患者身上获得了 47 类 OS 组织和相邻的正常组织。实时 PCR 用于测量 OS 组织和细胞系中的 circCRIM1 表达水平。OS细胞系的增殖、迁移和侵袭能力通过MTT测定、EDU测定、transwell迁移实验和transwell侵袭测定来测量。结果表明 circCRIM1 的表达在 OS 组织和细胞系中均显着降低。circCRIM1 表达降低与淋巴结转移相关，晚期FIGO分期，OS患者总生存率低。此外，结果表明circCRIM1可以减少OS细胞的迁移、侵袭和生长。进一步的机制研究表明，circCRIM1 作为 miR-513 的竞争性内源 RNA (ceRNA)，导致 OS 细胞增殖、迁移和侵袭的减少。总之，我们的数据揭示了 circCRIM1/miR-513 通路在 OS 细胞系的增殖、迁移和侵袭中的重要作用，并表明 circCRIM1 可能作为 OS 治疗的一个可能的治疗靶点。进一步的机制研究表明，circCRIM1 作为 miR-513 的竞争性内源 RNA (ceRNA)，导致 OS 细胞增殖、迁移和侵袭的减少。总之，我们的数据揭示了 circCRIM1/miR-513 通路在 OS 细胞系的增殖、迁移和侵袭中的重要作用，并表明 circCRIM1 可能作为 OS 治疗的一个可能的治疗靶点。进一步的机制研究表明，circCRIM1 作为 miR-513 的竞争性内源 RNA (ceRNA)，导致 OS 细胞增殖、迁移和侵袭的减少。总之，我们的数据揭示了 circCRIM1/miR-513 通路在 OS 细胞系的增殖、迁移和侵袭中的重要作用，并表明 circCRIM1 可能作为 OS 治疗的一个可能的治疗靶点。