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Pretreatment with 24-Epibrassinolide Synergistically Protects Root Structures and Chloroplastic Pigments and Upregulates Antioxidant Enzymes and Biomass in Na+-Stressed Tomato Plants
Journal of Plant Growth Regulation ( IF 3.9 ) Pub Date : 2021-09-03 , DOI: 10.1007/s00344-021-10481-5
Vitor Quintela Sousa 1 , Walter Fernando Serra Messias 1 , Ynglety Cascaes Pereira 1 , Breno Ricardo Serrão da Silva 1 , Elaine Maria Silva Guedes Lobato 1 , Allan Klynger da Silva Lobato 1 , Mohammed Nasser Alyemeni 2 , Parvaiz Ahmad 2, 3
Affiliation  

Salt stress reduces plant growth by negatively interfering with the division rate and cellular expansion, limiting the growth and development of the roots, stems, and leaves. 24-Epibrassinolide (EBR) is a molecule extracted from plant tissues and is a plant growth regulator with a high capacity to modulate tolerance to abiotic stresses. The objective of this study was to verify the possible improvements promoted by pretreatment with EBR in salt-stressed tomato plants, evaluating the variables related to root anatomy, photosynthetic pigments, antioxidant system, and biomass accumulation. The experiment comprised four treatments: two salt conditions (0 and 150 mM NaCl, described as Na+ (−) and Na+ ( +), respectively) and two concentrations of 24-epibrassinolide (0 and 100 nM EBR, described as EBR (−) and EBR ( +), respectively). EBR modulated the protection and vascularization of root structures, as demonstrated by the increases in epidermis thickness (12%) and metaxilem diameter (119%), respectively. This steroid relieved oxidative damage, which was clearly linked to elevated activities of superoxide ascorbate peroxidase (24%) and guaiacol peroxidase (31%). EBR also benefited photosynthetic pigments, reducing the degradation of chlorophylls. In addition, pretreatment with EBR favoured a higher biomass, which was due to positive effects on leaf and root tissues, including better performance of photosynthetic machinery.



中文翻译:

用 24-表油菜素内酯预处理协同保护根结构和叶绿素色素并上调 Na+ 胁迫番茄植物中的抗氧化酶和生物量

盐胁迫通过负面干扰植物的分裂率和细胞扩张来减少植物的生长,限制根、茎和叶的生长和发育。24-Epibrassinolide (EBR) 是一种从植物组织中提取的分子,是一种植物生长调节剂,具有很强的调节对非生物胁迫耐受性的能力。本研究的目的是验证 EBR 预处理在盐胁迫番茄植物中可能促进的改进,评估与根解剖、光合色素、抗氧化系统和生物量积累相关的变量。实验包括四种处理:两种盐条件(0 和 150 mM NaCl,描述为 Na + (-) 和 Na +(+)) 和两种浓度的 24-表油菜素内酯(0 和 100 nM EBR,分别描述为 EBR (-) 和 EBR (+))。EBR 调节了根结构的保护和血管化,如表皮厚度 (12%) 和后轴直径 (119%) 分别增加所证明的那样。这种类固醇减轻了氧化损伤,这显然与超氧化物抗坏血酸过氧化物酶 (24%) 和愈创木酚过氧化物酶 (31%) 的活性升高有关。EBR 还有益于光合色素,减少叶绿素的降解。此外,EBR 预处理有利于更高的生物量,这是由于对叶和根组织的积极影响,包括更好的光合机械性能。

更新日期:2021-09-04
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