Tissue engineering small diameter artificial blood vessel is an indispensable graft for repairing extremity arterial injury. Previous researches demonstrated that suitable porosity and matching mechanical properties are two keys for tubular tissue reconstruction. In this study, we fabricated a fabric reinforced small diameter tubular graft (PPTG) from PLGA fabric and three-dimensional porous PEEUU with a structure of the bionic extracellular matrix for transplanting in situ combined braiding and thermally induced phase separation (TIPS). The prepared PPTG presented smooth and dense inner surface, fluffy and porous grafts wall, and tubular PLGA fabric evenly dispersed PEEUU matrix, which provide mechanical support for PPTG. The mechanical tests and hemolysis results showed that PPTG possess matched mechanical properties with autologous blood vessels and stable blood compatibility. Six weeks after rabbits’ carotid artery defect replacement indicated that there were no blockage occurred for PPTG group. Histological staining exhibited positive expression of endothelial cells on the inner surface of PPTG and distinct smooth muscle in the wall of PPTG, which migrated from peripheral tissues and flowing blood. Therefore, this fabric reinforced small diameter tubular graft provides a solution for endothelialization and tissue regeneration and have a potential application in vascular trauma.

组织工程小直径人工血管是修复肢体动脉损伤不可缺少的移植物。先前的研究表明，合适的孔隙率和匹配的机械性能是管状组织重建的两个关键。在这项研究中，我们从 PLGA 织物和具有仿生细胞外基质结构的三维多孔 PEEUU 制造了一种织物增强的小直径管状移植物 (PPTG)，用于原位移植组合编织和热致相分离 (TIPS)。制备的PPTG内表面光滑致密，移植物壁蓬松多孔，管状PLGA织物均匀分散PEEUU基质，为PPTG提供了机械支撑。力学测试和溶血结果表明，PPTG具有与自体血管匹配的力学性能和稳定的血液相容性。兔颈动脉缺损置换术后6周，PPTG组未发生阻塞。组织学染色显示 PPTG 内表面内皮细胞阳性表达，PPTG 壁上有明显的平滑肌，从外周组织和流动的血液中迁移。所以，