Readthrough transcription caused by inefficient 3′-end cleavage of nascent mRNAs has emerged as a hallmark of the mammalian cellular stress response and results in the production of long noncoding RNAs known as downstream-of-gene (DoG)-containing transcripts. DoGs arise from around 10% of human protein-coding genes and are retained in the nucleus. They are produced minutes after cell exposure to stress and can be detected hours after stress removal. However, their biogenesis and the role(s) that DoGs or their production play in the cellular stress response are incompletely understood. We discuss findings that implicate host and viral proteins in the mechanisms underlying DoG production, as well as the transcriptional landscapes that accompany DoG induction under different stress conditions.

由新生 mRNA 的低效 3' 端切割引起的通读转录已成为哺乳动物细胞应激反应的标志，并导致长非编码 RNA 的产生，即含有基因下游 (DoG) 的转录本。 DoG 由大约 10% 的人类蛋白质编码基因产生，并保留在细胞核中。它们是在细胞受到压力后几分钟产生的，并且可以在压力消除后数小时内检测到。然而，它们的生物发生以及 DoG 或其产生在细胞应激反应中所起的作用尚不完全清楚。我们讨论了宿主和病毒蛋白在 DoG 产生机制中的发现，以及在不同应激条件下伴随 DoG 诱导的转录景观。