Fatty liver disease prevalently occurs in commercial postpartum dairies, resulting in a worldwide high culling rate because of their subsequent limitations of production and reproduction performance. Fatty liver-specific proteome and acetylome analysis revealed that energy metabolism suppression closely associated with mitochondrial dysfunction and inflammation activation were shown to be remarkable biological processes underlying the development of fatty liver disease, furthermore, acetylation modification of proteins could be one of the main means to modulate these processes. Twenty pivotal genetic factors/genes that differentially expressing and being acetylation modified in liver were identified and proposed to regulate the pathogenesis of fatty liver dairies. These proteins were confirmed to be differentially expressing in individual liver tissue, eight of which being validated via immunohistochemistry assay. This study provided a comprehensive proteome and acetylome profile of fatty liver of dairy cows, and revealed potential important biological processes and essential regulators in the pathogenesis of fatty liver disease. Expectantly, understanding the molecular mechanisms of the pathogenesis of fatty liver disease in dairies, as an animal model of non-alcoholic fatty liver disease (NAFLD) in human beings, which is a clinico-pathologically defined process associated with metabolic syndrome, could inspire and facilitate the development of efficacious therapeutic drugs on NAFLD.

中文翻译：

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蛋白质组分析确定了与线粒体功能和炎症激活相关的蛋白质，这些蛋白质对脂肪肝的发病机制至关重要

脂肪肝疾病普遍发生在商业产后奶牛场，由于其随后的生产和繁殖性能受到限制，导致全球范围内的高淘汰率。脂肪肝特异性蛋白质组和乙酰化组分析表明，与线粒体功能障碍和炎症激活密切相关的能量代谢抑制被证明是脂肪肝发生发展的显着生物学过程，此外，蛋白质的乙酰化修饰可能是预防脂肪肝的主要手段之一。调节这些过程。鉴定并提出了二十个在肝脏中差异表达和乙酰化修饰的关键遗传因素/基因，以调节脂肪肝乳制品的发病机制。这些蛋白质被证实在个体肝脏组织中差异表达，其中八种通过免疫组织化学测定进行验证。该研究提供了奶牛脂肪肝的全面蛋白质组和乙酰化谱，并揭示了脂肪肝发病机制中潜在的重要生物学过程和基本调节因子。期待了解乳制品脂肪肝发病机制的分子机制，作为人类非酒精性脂肪肝 (NAFLD) 的动物模型，这是与代谢综合征相关的临床病理学定义的过程，可以激发和促进对 NAFLD 的有效治疗药物的开发。该研究提供了奶牛脂肪肝的全面蛋白质组和乙酰化谱，并揭示了脂肪肝发病机制中潜在的重要生物学过程和基本调节因子。期待了解乳制品脂肪肝发病机制的分子机制，作为人类非酒精性脂肪肝 (NAFLD) 的动物模型，这是与代谢综合征相关的临床病理学定义的过程，可以激发和促进对 NAFLD 的有效治疗药物的开发。该研究提供了奶牛脂肪肝的全面蛋白质组和乙酰化谱，并揭示了脂肪肝发病机制中潜在的重要生物学过程和基本调节因子。期待了解乳制品脂肪肝发病机制的分子机制，作为人类非酒精性脂肪肝 (NAFLD) 的动物模型，这是与代谢综合征相关的临床病理学定义的过程，可以激发和促进对 NAFLD 的有效治疗药物的开发。