Endometrial cancer (EC) ranks as the most prevalent malignancy occurring in the female genital tract. Non-SMC condensin I complex subunit G (NCAPG), a mitotic associated chromosomal condensing protein, is reported to be frequently abnormally expressed in several tumors and plays a vital role in carcinogenesis. Our study aimed to explore the effect of NCAPG on cell proliferation and apoptosis in EC cells and to determine the underlying mechanism. Expression and survival data of NCAPG in EC tissues were analyzed by bioinformatics methods. Cell proliferation was evaluated by EdU and CCK-8 assays. Apoptosis was assessed by flow cytometry analysis. Expression of NCAPG, proliferating cell nuclear antigen (PCNA), Ki67, Bcl-2, Bax, active caspase-3, active β-catenin, and c-Myc were determined by western blotting. NCAPG was highly expressed in EC tissues and cells and predicted poor survival for EC patients. NCAPG knockdown inhibited cell proliferation and induced apoptosis in EC cells. Additionally, NCAPG knockdown inactivated the Wnt/β-catenin pathway in EC cells. Mechanistically, β-catenin overexpression blocked the tumorigenic effects of NCAPG in EC cells. In conclusion, NCAPG silencing inhibited cell proliferation and induced apoptosis in EC cells via inhibiting the Wnt/β-catenin pathway.

中文翻译：

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抑制 NCAPG 表达使 Wnt/β-catenin 信号失活以抑制体外子宫内膜癌细胞的生长

子宫内膜癌 (EC) 是女性生殖道中最常见的恶性肿瘤。据报道，非 SMC 凝聚素 I 复合亚基 G (NCAPG) 是一种与有丝分裂相关的染色体凝聚蛋白，在多种肿瘤中经常异常表达，并且在致癌作用中起着至关重要的作用。我们的研究旨在探讨 NCAPG 对 EC 细胞增殖和凋亡的影响，并确定其潜在机制。NCAPG 在 EC 组织中的表达和存活数据通过生物信息学方法进行分析。通过 EdU 和 CCK-8 测定评估细胞增殖。通过流式细胞术分析评估细胞凋亡。通过蛋白质印迹确定 NCAPG、增殖细胞核抗原 (PCNA)、Ki67、Bcl-2、Bax、活性 caspase-3、活性 β-连环蛋白和 c-Myc 的表达。NCAPG 在 EC 组织和细胞中高度表达，并预测 EC 患者的存活率低。NCAPG 敲低抑制细胞增殖并诱导 EC 细胞凋亡。此外，NCAPG 敲低使 EC 细胞中的 Wnt/β-catenin 通路失活。从机制上讲，β-连环蛋白过表达阻断了 NCAPG 在 EC 细胞中的致瘤作用。总之，NCAPG 沉默通过抑制 Wnt/β-catenin 通路抑制细胞增殖并诱导 EC 细胞凋亡。